In: Chemistry
A mixture contains 4 proteins with native molecular weights of: 120kDa, 100kDa, 25kDa, 15kDa is applied on a size exclusion chromatography column. The fractional range is 30-90kDa, the bed volume is 31mL, and the void volume is 10.5mL. Sketch a chromatograph with y-axis "arbitrary absorbance" and x-axis "volume of elution". X-axis should have ticks marking 10mL, 20mL, 30mL, and 40mL. Label the contents of each peak.