Question
In: Chemistry
A mixture contains 4 proteins with native molecular weights of: 120kDa, 100kDa, 25kDa, 15kDa is applied...
A mixture contains 4 proteins with native molecular weights of: 120kDa, 100kDa, 25kDa, 15kDa is applied on a size exclusion chromatography column. The fractional range is 30-90kDa, the bed volume is 31mL, and the void volume is 10.5mL. Sketch a chromatograph with y-axis "arbitrary absorbance" and x-axis "volume of elution". X-axis should have ticks marking 10mL, 20mL, 30mL, and 40mL. Label the contents of each peak.
Solutions
queen_honey_blossom answered 11 months agoRelated Solutions
A mixture of three proteins shown below was applied to an ion-exchange column with CM-Sepharose as...
A mixture of three proteins shown below was applied to an
ion-exchange column with CM-Sepharose as the stationary phase.
Predict the order of elution of the proteins at neutral pH,
assuming that the mixture was applied at low ionic strength and
eluted with buffers of increasing ionic strength. Explain.
Cytochrome c (12.0 kDa, pI = 10.6)
Phosphofructokinase 1 (34.1 kDa, pI = 6.64)
Bovine serum albumin (66.5 kDa, pI = 4.70)
4 levels of proteins? Describe with detail the molecular configuration of each level. What molecular and...
4 levels of proteins? Describe with detail the molecular
configuration of each level. What molecular and environmental items
are required to ensure that proteins are functional?
You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1...
You have a mixture of the proteins listed below.
Protein
pI
Molecular Weight (kDa)
A
3.1
265
B
6.9
93
C
10.3
96
D
7.1
43
E
8.6
189
1) You load the protein mix onto a cation exchange column at pH
5. Next, you apply a "washing" step by passing
through buffer at pH 5. Finally, for your elution
step, you apply a pH gradient starting from pH 2.0 to pH
13.0. (A gradient buffer system allows you to...
You have mixture of proteins with the following properties. Which of these proteins would be eluted...
You have mixture of proteins with the following properties.
Which of these proteins would be eluted last from an anion-exchange
column when the column is developed with 1.0M NaCl in pH 7.0
buffer. Note, that the anion-exchange column was equilibrated with
the pH 7.0 buffer before use.
A. MW = 18,500, pI = 10.2
B. MW = 28,000, pI = 7.0
C. MW = 62,000, pI = 3.9 CORRECT ANSWER
D. MW = 9,000, pI = 5.8
E. MW =...
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only liquid. The total pressure is then slowly reduced to a
pressure of 60 Torr. Calculate the composition of liquid phase and
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The weights of the native North American Blue Jay have a mean of 18.4 ounces with...
The weights of the native North American Blue Jay have a
mean of 18.4 ounces with a standard deviation of 3.6 ounces. The
distribution can be considered normal. What is the 2.5th percentile
of all weights?
A. 11.344
B. 14.800
C. 13.792
D. 12.478
For the data in problem 7, what is the 97.5th percentile
of all weights? A. 25.456
B. 27.688
C. 24.322
D. Can’t tell from the given data
A population of fruit flies has 30% black and...
A mixture contains only and . A 4.51-g sample of the mixture is dissolved in water...
A mixture contains only and . A 4.51-g sample of the mixture is
dissolved in water and an excess of is added, producing a
precipitate of . The precipitate is filtered, dried, and weighed.
The mass of the precipitate is 1.06 g. What is the mass percent of
in the sample?
%
An aqueous solution contains a mixture of .175 M HF (Ka = 7.2 x 10-4) and...
An aqueous solution contains a mixture of .175 M HF
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The movement of molecular motors depends on the conformational changes that the proteins undergo as they...
The movement of molecular motors depends on the conformational
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a nonhydrolyzable form of ATP to myosin, at what point would you
block its movement? How is this similar to or different from adding
a nonhydrolyzable form of ATP to kinesin?
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