Question

 

7) What are the Coenzyme A structural features

8) Oxidation of fats and synthesis of fats contain a molecule with common theme

9) Oxidative deamination of glutamate and transamination reactions in general

Answer 1

7) A coenzyme is made up of three components:

a) 3’-phosphorylated ATP

b) Vitamin B5 or pantothenate

c) Cysteine

8) CoA is used in fatty acid oxidation while Acyl carrier protein (ACP) is used as a cofactor in fatty acid biosynthesis. Both of them are similar in structure, they have pantothenate and cysteine

9) Oxidative deamination is the process of releasing oxidatively deaminating an amino acid to form ?-ketoglutarate (an ?-keto acid). This releases an ammonium ion while reducing a mole of NADP/ mole of glutamate. This is carried out by glutamate dehydrogenase.

Transamination is done by a different class of enzymes known as aminotransferase that is dependent on Vitamin B6 for the reaction to proceed. In this reaction, an amino group is transferred from an amino acid to ?-ketoglutarate. The product of the reaction is glutamate and an ?-keto acid. This reaction is not coupled with the release of ammonium ions or reduced electron carrier molecules.