Question

Enzyme Used	Lysozyme
Thermal Denaturation
Temp (Celsius)	Abs (325 nm)
40	0.106
50	0.086
60	0.109
65	0.161
70	0.409
75	2.06
80	2.173
90	0.821
Urea Denaturation
M of urea	Abs (325 nm)
0	0.464
1	0.07
2	0.056
4	0.05
6	0.035
8	0.028

Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find ΔH, ΔS and ΔG(T=75) (mention any assumptions you made) and What is the Tm for each protein

Answer 1

Solution:

Delta G = -RT ln K

From data of at 65 degree celcius having In K = -6.57

Delta G = -RT ln K = -18462.56 J mol-1 K-1

FROM THE GIVEN EQUATION

Delta G = Delta H + Delta TS

Tm for each protein

From the graph of K Vs. T-1

Tm = 1/ 0.0133 = 75.18 oC