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You have just isolated a new strain of Gram negative bacteria that can catabolize the sugar...
You have just isolated a new strain of Gram negative bacteria that can catabolize the sugar aspartame. Genome comparisons to all other strains don't provide any insight into the identity of this pathway. 1) Describe an experiment to identify the pathway coding for aspartame catabolism 2) Describe an experiment to move this pathway into E. coli 3) Given what we learned in class, describe how this pathway is likely positively regulated and where promoter sequence would be 4) Describe an experiment to phenotypically confirm how this pathway is regulated using a SCREEN involving styrene catabolism 5) Describe an experiment to phenotypically confirm how this pathway is regulated using a SELECTION involving LacZ
Please list relevant strain/plasmid names, or draw/describe your own custom plasmids for these experiments
Solutions
Amanda K answered 2 years agoRelated Solutions
You have just isolated a new strain of Gram negative bacteria that can catabolize the sugar...
You have just isolated a new strain of
Gram negative bacteria that can catabolize the sugar aspartame.
Genome comparisons to all other strains don't provide any insight
into the identity of this pathway. 1) Describe an experiment to
identify the pathway coding for aspartame catabolism 2) Describe an
experiment to move this pathway into E. coli 3) Given what
we learned in class, describe how this pathway is likely repressed
and where operator sequence would be 4) Describe an experiment...
You just isolated a new strain of mutant mice, and preliminary mating suggest that the new...
You just isolated a new strain of mutant mice, and preliminary
mating suggest that the new mutant phenotype is not inherited in an
autosomal fashion. Circumstantial evidence seems to indicate that
the mutant phenotype may be following either an X-linked dominant
or a mitochondrial-type inheritance pattern.
a.) What two informative crosses would you set up to distinguish
between these two possibilities?
b.) How would the results distinguish between the two
possibilities?
Can AMPs that are mainly used for gram-negative bacteria be used on gram-positive as well?
Can AMPs that are mainly used for gram-negative bacteria be used
on gram-positive as well?
You have just isolated bacteria from the back of your throat. How can you most quickly...
You have just isolated bacteria from the back of your throat.
How can you most quickly determine if you have streptococci or
staphylococci?
A.
Hemolysis on blood agar
B.
Gram stain
C.
Coagulase test
D.
Catalase test
A positive phenylalanine test results in a ______color in the
tube.
red
yellow
green
purple
What type of medium is used in the Kirby-Bauer method?
A.
Chocolate agar
B.
Mueller-Hinton
C.
Tryptic soy agar
D.
Eosin methylene blue agar
What two sugars must...
Explain the differences between Gram positive bacteria wall and Gram negative bacteria cell wall.
Explain the differences between Gram positive bacteria
wall and Gram negative bacteria cell wall.
4. After performing a Gram stain on a Gram negative bacteria, the specimen shows purple bacteria....
4. After performing a Gram stain on a Gram negative bacteria,
the specimen shows purple bacteria. Which of the following best
explains why this occurred? A. Crystal violet was not used B.
Decolorizer was not used C. The specimen was not heat-fixed D.
Safranin was not used
5. Which of the following pieces of evidence could support the
endosymbiotic theory if found in some eukaryotes? A. Presence of
80S ribosomes B. The presence of formylated methionine C. Genes
that allow...
what is the bacteria of that is gram negative, acid fast negative, and endospore forming? there...
what is the bacteria of that is gram negative, acid fast
negative, and endospore forming? there is growth on blood and
macconkey not MSA.
options:
1, salmonella pulloram
2, enterobacter aerogenes
3, strep. mutans
4, micrococcus luteus
5, mycobacterium smegmatis
6, bacillus subtilis
7, staph. aureus
its
actually non-endosporing
OUTLINE the differences in the envelope structures of Gram-positive and Gram-negative bacteria. You may use diagrams...
OUTLINE the differences in the envelope structures of
Gram-positive and Gram-negative bacteria. You may use diagrams or a
table.
Describe the behavior of chemotaxis in bacteria. Make sure to
mention how the flagella contributes and how they can move toward
good things and away from bad things.
DESCRIBE the Endosymbiotic Theory and OUTLINE the evidence to
support
Choose one antibiotic that you would expect to show different effects on Gram-positive and Gram-negative bacteria....
Choose one antibiotic that you would expect to show different
effects on Gram-positive and Gram-negative bacteria. Which
bacterium is more susceptible? Why do you think this might be the
case?
For each type of cell wall in bacteria (gram positive vs. gram negative ) describe the...
For
each type of cell wall in bacteria (gram positive vs. gram negative
) describe the unique components present in each and list how each
layer of the cell wall is connected together ( what molecules
accomplish this)?
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