Question

In: Biology

1. You are investigating the genetics of two independently derived true-breeding lines of blackfish that are...

1. You are investigating the genetics of two independently derived true-breeding lines of blackfish that are both albino. When you crossed females from line 1 with males from line 2, all offspring were wildtype black (N=500). This means that:

		The albino allele is homozygous lethal.
		In both lines, albinism is caused by a gene in the mitochondrial genome.
		The albino allele is completely dominant to the black allele.
		Albinism in these lines is caused by alleles at two different loci.
		Albinism in these lines is caused by alleles at the same locus.

You perform a co-transformation experiment with your favorite species of bacteria and get the following results.

Assume 0% co-transformation for any combinations of genes that are not in this table.

Genes	Co-transformation frequency (%)
aoN and zlM	0.87
eiA and aoN	0.15
zlM and bpC	0.61
rkJ and eiA	1.24
gzW and rkJ	0.26

Which of the following pairs of genes is closest together on the bacterial chromosome?

		zlM and bpC
		gzW and bpC
		rkJ and eiA
		eiA and aoN
		gzW and aoN
		aoN and zlM

Which of the following genotypes is transcription of the lac operon regulated by the presence or absence of allolactose?

		lacI+ lacP+ lacO+ lacZ-
		lacI^S lacP+ lacO+ lacZ+
		lacI+ lacP+ lacO^C lacZ+
		lacI+ lacP- lacO+ lacZ+
		lacI- lacP+ lacO+ lacZ+

Which of the following genotypes is transcription of the lac operon regulated by the presence or absence of allolactose?

		lacI+ lacP+ lacO+ lacZ-
		lacI^S lacP+ lacO+ lacZ+
		lacI+ lacP+ lacO^C lacZ+
		lacI+ lacP- lacO+ lacZ+
		lacI- lacP+ lacO+ lacZ+

Although operons are not common in eukaryotes, eukaryotic genes may be activated by the same stimulus. Which of the following regulatory DNA sequences makes this coordinated gene expression possible?

		Kozak sequence
		Response element
		Silencer
		Core promoter
		Insulator

Solutions

Expert Solution

Question1:

Answer: Albinism in these lines is caused by alleles at two different loci.

If albino allele homozygous lethal then both parents will already die before crossing. If this is mitochondrial genes then all male and half female should be albino. Albinism in these lines is from alleles at two different loci. So they can be albino in offspring if they came from same loci.

Question 2:

Answer: rkJ and eiA

Co-transformation is higher in closed genes. rkJ and eiA genes have maximum co-transformation frequency.

Question 3:

Answer: lacI+ lacP+ lacO+ lacZ-

lacI- = Produce defective repressor, lac operon always On

lacIs = Produced defective repressor, inducer can’t bind so always Off

LacOs = repressor never bind, always On

lacP- = promotor never bind, so not transcription

Question 4: Repeated of question 3

Answer: lacI+ lacP+ lacO+ lacZ-

lacI- = Produce defective repressor, lac operon always On

lacIs = Produced defective repressor, inducer can’t bind so always Off

LacOs = repressor never bind, always On

lacP- = promotor never bind, so not transcription

Question 5:

Answer: Kozak sequence

Kozak sequence, motif, which involved in the initiation of translation. It confirms the correctness of translation. Response element present in promotor which help in transcription. Silence genes involved in switch off of transcription. They present in repressor gene. Core promoter help in transcription. Insulator regulate transcription.

gladiator answered 1 year ago

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