In: Anatomy and Physiology
You are a researcher interested in studying Schwann cell development. It is known from prior work that Schwann cells express the protein Sox10. You decide to create a transgenic fish line to label the Schwann cells. Explain how you would create the transgenic embryos. Be as detailed as possible.
* Transgenic embryos are created by genetic manipulation of host species that carry exogenous genetic material or genes from other species in their genome.
* This technique is used to introduce new genes or genes of interest into strains of laboratory to study function and pathology regarding specific gene.
* Fish is the first genetically modified animal to be used for consumption in humans.
* The fish is essentially an Atlantic salmon with single gene complex inserted, a growth hormone regulating gene from chinook salmon with a promoter sequence.
* The aim is to introduce new trait to fish that before does not occur in nature as a species. This process is called trans genesis.
* Genetically modified fish are developed as environmental pollutant sentinels and also used in aquaculture for production of food.
Transgenic fish are used in research centers
* Indicators of aquatic pollutants.
* used in functional genomic studies.
* Development in biomedical use as reactors for important proteins, used in cardiovascular diseases etc.