Which of the following proteins does not bind GTP? EF-Tu EF-G IF-2 Phosphohexose Isomerase

Which of the following proteins does not bind GTP?

		EF-Tu
		EF-G
		IF-2
		Phosphohexose Isomerase

Expert Solution

Answer- Phosphohexose isomerase

Explanation- phosphohexose isomerase does not have GTP binding domain and activity of isomerase does not depending on GTP. Isomerase enzyme converts one hexose sugar into another hexose sugar by the internal rearrangement of atoms without use of GTP. Phosphohexose isomerase does not binds to GTP.

Proteins that binds to GTP-

EF-Tu - EF-Tu is the translation elongation factor in prokryotes. EF-Tu is G-protein which can bind with GTP and uses GTP for it's function. EF-Tu-GTP binds to charged tRNA helps to translocate tRNA to A-site and when tRNA forms interaction with mRNA codon then GTPase action of EF-Tu hydrolyze GTP to GDP and due to this EF-Tu conformation change ocuurs and it leaves from ribosome.

EF-G - EF-G is the translation elongation factor in prokaryotes. EF-G has GTP binding domain and helps to translocate tRNA from A site to P site and Psite to E site. When ribosomal GTPase hydrolyze GTP to GDP then EF-G-GDP leaves complex and tRNA translocation occurs.

IF-2 - IF-2 is the initiation factor required in translation initiation in prokaryotes. When IF-2 is bound with GTP then it binds with small ribosomal subunit and then initiator tRNA binds in P-site of ribosome. Then large ribosomal subunit can bind the complex and large ribosomal subunit hydrolyzes GTP into GDP and after this conversion IF-2 gets released from small subunit and large ternary initiation complex is completed.

gladiator answered 2 years ago

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