Question

Chromatography explained in details highlighting important things to know for test.

Answer 1

Usually this chromatography is a important biophysical technique used for separation, identification and purification of components.

It is mainly used for the separation of mixtures, and the mixture in which fluid is dissolved is mobile phase and which carries it through a structure holding another material is stationary phase.

There are many types of chromatography like paper chromatography, Thin layer chromatography, gel chromatography, column chromatography, ion exchange chromatography, HPLC etc

In almost all chromatography's the following factors are relevant those are Mobile phase composition, Stationary phase, Pressure, Temperature, Flow rate, Concentration of analyte.

Suppose if in chromatography there is any particulate stationary phase then we need consider the following factors i.e Pore size of stationary phase, Surface area of stationary phase, Particle size.

In liquid chromatography, mobile phase pH is important parameter.

In ion exchange chromatography, ionic type and ionic charge and type of ion exchange material and stationary phase pH are important.

In gas chromatography pressure, temperature and analyte vapor pressure are important parameter.

One more important factor is retention factor it is used for a particular material and it gives the ratio of the distance the spot moved above the origin to the distance the solvent front moved from origin.