Spectrometry. The absorbance A of a solution
is defined as
A =
log10(I0/I)
in which I0 is the incident-light intensity
and I is the transmitted-light intensity. The absorbance
is related to the molar absorption coefficient (extinction
coefficient) ε (in M–1 cm–1), concentration
c (in M), and path length l (in cm) by
A = εlc
The absorption coefficient of myoglobin at 580 nm is 15,000
M–1 cm–1. What is the absorbance of a 1 mg
ml–1 solution across a 1-cm...