Spectrometry. The absorbance A of a solution is defined as A = log10(I0/I) in which I0...

Spectrometry. The absorbance A of a solution is defined as

A = log₁₀(I₀/I)

in which I₀ is the incident-light intensity and I is the transmitted-light intensity. The absorbance is related to the molar absorption coefficient (extinction coefficient) ε (in M^–1 cm^–1), concentration c (in M), and path length l (in cm) by

A = εlc

The absorption coefficient of myoglobin at 580 nm is 15,000 M^–1 cm^–1. What is the absorbance of a 1 mg ml^–1 solution across a 1-cm path? What percentage of the incident light is transmitted by this solution?

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queen_honey_blossom answered 1 year ago

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