Question
In: Chemistry
Spectrometry. The absorbance A of a solution is defined as A = log10(I0/I) in which I0...
Spectrometry. The absorbance A of a solution is defined as
A = log10(I0/I)
in which I0 is the incident-light intensity and I is the transmitted-light intensity. The absorbance is related to the molar absorption coefficient (extinction coefficient) ε (in M–1 cm–1), concentration c (in M), and path length l (in cm) by
A = εlc
The absorption coefficient of myoglobin at 580 nm is 15,000 M–1 cm–1. What is the absorbance of a 1 mg ml–1 solution across a 1-cm path? What percentage of the incident light is transmitted by this solution?
Solutions
queen_honey_blossom answered 2 years agoRelated Solutions
The formula for measuring sound intensity in decibels D is defined by the equation D = 10log(I/I0) where I is the intensity of the sound in watts per square meter and I0 = 10−12 is the.
The formula for measuring sound intensity in decibels D is defined by the equation D = 10log(I/I0) where I is the intensity of the sound in watts per square meter and I0 = 10−12 is the lowest level of sound that the average person can hear. How many decibels are emitted from a rock concert with a sound intensity of 4.7 · 10−1 watts per square meter?
Given the pH and absorbance of a solution. What equation would i use to find the...
Given the pH and absorbance of a solution. What equation would i
use to find the
1.equilibrium concentration of the base? 2. equilibrium
concentration of the acid?
Explain the relationship between light absorbance and solution concentration for a solution which obeys the Beer-Lambert...
Explain the relationship between light absorbance and solution
concentration for a solution which obeys the Beer-Lambert law.
how do i draw a table that shows wavelength and absorbance data for stock solution and...
how do i draw a table that shows wavelength and absorbance data
for stock solution and brass solution?
A solution containing a mixture of the compounds X and Y had an absorbance of 1.00...
A solution containing a mixture of the compounds X and Y had an
absorbance of 1.00 at 443 nm and an absorbance of 0.861 at 520 nm
when measured with a 1.00 cm cell. The molar absorptivities (ε) of
X and Y at each wavelength are shown in the table below. 443 16410
3871. 520 3993 6425
For your indicator, what is the wavelength of maximum absorbance for the pH < 4.00 solution?...
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima? This is the
procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204
Suppose you prepared a solution with an absorbance that was too high and off the scale...
Suppose you prepared a solution with an absorbance that was too
high and off the scale of your calibration curve (Assume the
Spec-20 instrument was properly calibrated). What would be the most
likely problem? What would you do to reduce the absorbance? Explain
why it should be reduced.
The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument...
The absorbance of a cationic iron(II) sample solution was measured
in a spectrophotometer, but the instrument returned an error
because the absorbance was too high. The sample was then diluted by
using a pipette to take 100.0
μL
of the sample and injecting it into a cuvette already containing
2.00
mL
of water (total volume is 2.00
mL
+ 100.0
μL).
The absorbance value of the diluted solution corresponded to a
concentration of 7.71×10−6
M . What was the concentration...
Given the absorbances and concentrations of NO2-, calculate the absorbance of a 7.0μM solution. ( Hint:...
Given the absorbances and concentrations of
NO2-, calculate the absorbance of a 7.0μM
solution. ( Hint: Need to construct a Beer's Law Graph) Write
answer to 3 decimal places.
Concentration (μM)
200
100
50
25
15
10
Absorbance
1.13
0.58
0.31
0.14
0.1
0.05
For your indicator, what is the wavelength of maximum absorbance for the pH < 4.00 solution?...
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima?
This is the procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204
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