Question

In: Chemistry

Explain how metabolic pathways are regulated via regulatory enzymes? How would you determine potential regulation reactions,...

Explain how metabolic pathways are regulated via regulatory enzymes?

How would you determine potential regulation reactions, given free energy changes for reactions?

Solutions

Expert Solution

Enzymes can be regulated by changing the activity of a preexisting enzyme or changing the amount of an enzyme.

A. Changing the activity of a pre-existing enzyme: The quickest way to modulate the activity of an enzyme is to alter the activity of an enzyme that already exists in the cell. The list below, illustrated in the following figure, gives common ways to regulate enzyme activity

  1. Substrate availability: Substrates (reactants) bind to enzymes with a characteristic affinity (characterized by a dissociation constant) and a kinetic parameter called Km (units of molarity). If the actual concentration of a substrate in a cell is much less than the Km, the activity of the enzyme is very low. If the substrate concentration is much greater than Km, the enzyme active site is saturated with substrate and the enzyme is maximally active.  
  2. Product inhibition:   A product of an enzyme-catalyzed reaction often resembles a starting reactant, so it should be clear that the product should also bind to the activity site, albeit probably with lower affinity. Under conditions in which the product of a reaction is present in high concentration, it would be energetically advantageous to the cell if no more product was synthesized. Product inhibition is hence commonly observed.   Likewise it be energetically advantageous to a cell if the end product of an entire pathway could likewise bind to the initial enzyme in the pathways and inhibit it, allowing the whole pathway to be inhibited.  
  3. Allosteric regulation: As many pathways are interconnected, it would be optimal if the molecules of one pathway affected the activity of enzymes in another interconnected pathway, even if the molecules in the first pathway are structurally dissimilar to reactants or products in a second pathway. Molecules that bind to sites on target enzymes other than the active site (allosteric sites) can regulate the activity of the target enzyme. These molecules can be structurally dissimilar to those that bind at the active site. They do so my conformational changes which can either activate or inhibit the target enzyme's activity.
  4. pH and enzyme conformation: Changes in pH which can accompany metabolic process such as respiration (aerobic glycolysis for example) can alter the conformation of an enzyme and hence enzyme activity. The initial changes are covalent (change in protonation state of the protein) which can lead to an alteration in the delicate balance of forces that affect protein structure.
  5. pH and active site protonation state: Changes in pH can affect the protonation state of key amino acid side chains in the active site of proteins without affecting the local or global conformation of the protein. Catalysis may be affected if the mechanism of catalysis involves an active site nucleophile (for example), that must be deprotonated for activity.
  6. Covalent modification: Many if not most proteins are subjected to post-translational modifications which can affect enzyme activity through local or global shape changes, by promoting or inhibiting binding interaction of substrates and allosteric regulators, and even by changing the location of the protein within the cell. Proteins may be phosphorylated, acetylated, methylated, sulfated, glycosylated, amidated, hydroxylated, prenylated, myristolated, often in a reversible fashion. Some of these modifications are reversible. Regulation by phosphorylation through the action of kinases, and dephosphorylation by phosphates is extremely common. Control of phosphorylation state is mediated through signal transduction process starting at the cell membrane, leading to the activation or inhibition of protein kinases and phosphatases within the cell.

B. Changing the amount of an enzyme: Another and less immediate but longer duration method to modulate the activity of an enzyme is to alter the activity of an enzyme that already exists in the cell. The list below, illustrated in the following figure, shows way in which enzyme concentration is regulated.

  1. Alternation in transcription of enzyme's gene: Extracellular signal (hormones, neurotransmitters, etc) can lead to signal transductions responses and ultimate activation or inhibition of the transcription of the gene for a protein enzyme. These changes result from recruitment of transcription factors (proteins) to DNA sequences that regulate transcription of the enzyme gene.
  2. Degradation of messenger RNA for the enzyme: The levels of messenger RNA for a protein will directly determin the amount of that protein synthesized. Small inhibitor RNAs, derived from microRNA molecules transcribed from cellular DNA, can bind to specific sequences in the mRNA of a target enzyme. The resulting double-stranded RNA complex recruits an enzyme (Dicer) that cleaves the complex with the effect of decreasing translation of the protein enzyme from its mRNA.
  3. Co/Post translational changes: Once a protein enzymes is translated from its mRNA, it can undergo a changes to affect enzyme levels. Some proteins are synthesized in a "pre"form which must be cleaved in a targeted and limited fashion by proteases to active the protein enzyme. Some proteins are not fully folded and must bind to other factors in the cell to adopted a catalytically active form. Finally, fully active protein can be fully proteolyzed by the proteasome, a complex within cells, or in lysosomes, which are organelles within cells containing proteolytic enzymes.

Related Solutions

Explain how metabolic pathways are regulated via regulatory enzymes? How would you determine potential regulation reactions,...
Explain how metabolic pathways are regulated via regulatory enzymes? How would you determine potential regulation reactions, given free energy changes for reactions?
Explain the statement, “Thermodynamics determines the direction and regulatory capacity of metabolic pathways”
Explain the statement, “Thermodynamics determines the direction and regulatory capacity of metabolic pathways”
The lac operon is regulated via positive and negative regulation. Explain how each mechanism works on...
The lac operon is regulated via positive and negative regulation. Explain how each mechanism works on this operon, and under which circumstances will the operon be turned on. Include the definition of an operon 2. What are the advantages to a microbe of having inducible enzymes? What is the function of toluene in the experiment where you induced enzyme function?
Write an short essay abouy How can enzymes speed up the metabolic reactions One A4 page...
Write an short essay abouy How can enzymes speed up the metabolic reactions One A4 page No copy-paste from the internet(own sentences)
Explain the regulation of forced breathing.  Provide all structures as well as any chemical reactions you would...
Explain the regulation of forced breathing.  Provide all structures as well as any chemical reactions you would expect to be involved.  
Vitamins serve as co-enzymes in many biochemical reactions. What vitamins (co-enzymes) would you supply bacteria and...
Vitamins serve as co-enzymes in many biochemical reactions. What vitamins (co-enzymes) would you supply bacteria and which you would remove from the medium to prevent accumulation of fat by bacteria.
Physiology -Discuss the three metabolic pathways that supply ATP for muscle contraction -Explain how navigational abilities...
Physiology -Discuss the three metabolic pathways that supply ATP for muscle contraction -Explain how navigational abilities promote reproductive success. Provide an example.
In a step by step manner, explain how the voltage regulated channels produce an action potential.
In a step by step manner, explain how the voltage regulated channels produce an action potential.
Describe how sugar is catabolized to pyruvate via glycolysis. Explain how these catabolic reactions generate ATP...
Describe how sugar is catabolized to pyruvate via glycolysis. Explain how these catabolic reactions generate ATP and NADH.
You are one of the potential investors in a business . Explain how you would go...
You are one of the potential investors in a business . Explain how you would go about evaluating the proposed investment. Use at least three different methods of evaluating investments. Describe the three different methods, and explain which one of these methods used is the best for this particular decision. Motivate your answer. Describe any potential negative issues of the chosen method which you should be aware of. Answer must be between 1500-2000 word counts
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT