Question

In: Biology

The lac operon is regulated via positive and negative regulation. Explain how each mechanism works on...

The lac operon is regulated via positive and negative regulation. Explain how each
mechanism works on this operon, and under which circumstances will the operon be
turned on. Include the definition of an operon
2. What are the advantages to a microbe of having inducible enzymes? What is the
function of toluene in the experiment where you induced enzyme function?

Solutions

Expert Solution

Ans1-)Operon is defined as the group of genes generally the structural genes are present on the chromosome and thus these structural genes are transcribed together.
Operon generally consist of regulator, operator and structural genes.
1. Absence of Lactose:
When the Inducer which is Lactose is absent, then the repressor gene will produce the repressor protein which is active and thus bind to the operator region. And thereby preventing the RNA polymerase to transcribe the genes and no protein is being formed.


2. Presence of Lactose:
When the inducer which is a Lactose is present, will fit and will inactivate the repressor protein. Thus, the repressor protein cannot bind to the operator. Hence, the RNA polymerase can now sit on the operator region and will bind to the promoter region and will transcribe the genes.
Gene Z encodes for Beta- Galactosidase
Gene Y encodes for Permease
Gene A encodes for Transacetylase.
Operon is turned on when the Inducer(Lactose) is present.

Ans 2-) Advantages of microbe
1. Energy is saved as when there is no use of the enzyme it will not make it.
2. Also, cost of resource is very less. The expression of inducible enzyme occurs only under the condition which require it.

Toluene will decrease the cell viability. Also, turbidity will be reduced.


Related Solutions

Explain how the lac operon is subject to positive and negative control.
Explain how the lac operon is subject to positive and negative control.
Explain how the lac operon is subject to positive and negative control.
Explain how the lac operon is subject to positive and negative control.
Explain how gene regulation occurs using the lac operon as an example.
Explain how gene regulation occurs using the lac operon as an example.
Explain how gene regulation occurs using the lac operon as an example
Explain how gene regulation occurs using the lac operon as an example
A)How is the lac operon regulated if the bacteria is put into an environment where there...
A)How is the lac operon regulated if the bacteria is put into an environment where there is excess amount of lactose and glucose at the same time. (10 pts) B)From the previous question, how would the outcome change if the glucose and lactose amounts are very low? (10 pts) C)Where would the lac repressor be bound in E. Coli bacteria that is growing in low glucose and high lactose? (7pts)
How is the lac operon regulated if the bacteria is put into an environment where there...
How is the lac operon regulated if the bacteria is put into an environment where there is excess amount of lactose and glucose at the same time. (10 pts) 7) From the previous question, how would the outcome change if the glucose and lactose amounts are very low? (10 pts)
Investigating the negative regulation of the lac operon by allolactose, consider the following merozygote experiment: I...
Investigating the negative regulation of the lac operon by allolactose, consider the following merozygote experiment: I – O + Z + / F' I S O C Z –. Which statement correctly describes the regulation of ß-galactosidase synthesis in this merodiploid cell? Note that ß-galactosidase expression can come from either the chromosome or the plasmid. Without lactose ß-galactosidase synthesis is ON; with lactose it is ON Without lactose ß-galactosidase synthesis is ON; with lactose it is OFF Without lactose ß-galactosidase...
6) How is the lac operon regulated if the bacteria is put into an environment where...
6) How is the lac operon regulated if the bacteria is put into an environment where there is excess amount of lactose and glucose at the same time. (10 pts)
6) How is the lac operon regulated if the bacteria is put into an environment where...
6) How is the lac operon regulated if the bacteria is put into an environment where there is excess amount of lactose and glucose at the same time. (10 pts) 7) From the previous question, how would the outcome change if the glucose and lactose amounts are very low? (10 pts)
The lac operon is just one specific example of an operon that is regulated both negatively,...
The lac operon is just one specific example of an operon that is regulated both negatively, by binding of a repressor, and positively by catabolite repression.  Describe another example of this type, providing details of the ligands that positively and negatively regulate transcription of the operon, and briefly describe the genes in the operon and the molecular logic of the specific operon you describe.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT