Write a about bacterial identification by biochemical & molecular methods?

Write a about bacterial identification by biochemical & molecular methods?

Expert Solution

Bacterial cell can be analyzed through morphological (shape and size), biochemical and molecular methods. Gram staining, biochemical activities like oxidase, enzyme catalase, enzyme urease, beta galactosidase, and their grown on different culture medium like blood agar (type of hemolysis, alpha, beta or gamma), MSA, optochin and bacitracin sensitivity can identify bacterial organism. Molecular characterization can be employed to when both organism exhibits similar morphological and biochemical properties and in this method we can use DNA sequencing (by using specific sequences) to compare two. Molecular methods are more important to identify pathogenic strain from the non-pathogenic strain and it is quick method being used in clinical settings. Ribosomal gene is a marker in the bacteria and it shows variation in different bacterial, so this method further can be used for their confirmation. In eukaryotic 18S ribosomal RNA present whereas in bacterial cell 16S ribosomal RNA present.

gladiator answered 2 years ago

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