Question

In: Biology

Write a about bacterial identification by biochemical & molecular methods?

Write a about bacterial identification by biochemical & molecular methods?

Solutions

Expert Solution

Bacterial cell can be analyzed through morphological (shape and size), biochemical and molecular methods. Gram staining, biochemical activities like oxidase, enzyme catalase, enzyme urease, beta galactosidase, and their grown on different culture medium like blood agar (type of hemolysis, alpha, beta or gamma), MSA, optochin and bacitracin sensitivity can identify bacterial organism. Molecular characterization can be employed to when both organism exhibits similar morphological and biochemical properties and in this method we can use DNA sequencing (by using specific sequences) to compare two. Molecular methods are more important to identify pathogenic strain from the non-pathogenic strain and it is quick method being used in clinical settings. Ribosomal gene is a marker in the bacteria and it shows variation in different bacterial, so this method further can be used for their confirmation. In eukaryotic 18S ribosomal RNA present whereas in bacterial cell 16S ribosomal RNA present.


Related Solutions

A biochemical engineer isolates a bacterial gene fragment and dissolves a 13.0 mg sample of the...
A biochemical engineer isolates a bacterial gene fragment and dissolves a 13.0 mg sample of the material in enough water to make 39.7 mL of solution. The osmotic pressure of the solution is 0.340 torr at 25°C. (a) What is the molar mass of the gene fragment? (b) If the solution density is 0.997 g/mL, how large is the freezing point depression for this solution (Kf of water=1.86°C/m)?
A biochemical engineer isolates a bacterial gene fragment and dissolves a 17.0 mg sample of the...
A biochemical engineer isolates a bacterial gene fragment and dissolves a 17.0 mg sample of the material in enough water to make 38.9 mL of solution. The osmotic pressure of the solution is 0.340 torr at 25
What is the molecular basis of Congenital Adrenal Hyperplasia? (what is abnormal at the cellular, biochemical,...
What is the molecular basis of Congenital Adrenal Hyperplasia? (what is abnormal at the cellular, biochemical, or molecular level?) (250 words or less)
Compare and contrast classical and serological identification of bacterial, viral, fungal and parasitic human pathogens
Compare and contrast classical and serological identification of bacterial, viral, fungal and parasitic human pathogens
15. The internationally recognized reference for bacterial classification and identification is called Bergey’s Manual for Systematic...
15. The internationally recognized reference for bacterial classification and identification is called Bergey’s Manual for Systematic Bacteriology True False 27. Heat-fixation is performed in slide preparation in order to _________________________________.         Change the morphology of the organisms on the slide         Encourage bacterial growth on the slide         Inactivate and adhere microorganism placed on the slide         Protect the bacteria by hardening them to prevent destruction by the staining procedure 30. New approaches to antimicrobial therapy...
Explain the routine laboratory techniques and tests (microscopy, bacterial culture media and conditions, biochemical tests and...
Explain the routine laboratory techniques and tests (microscopy, bacterial culture media and conditions, biochemical tests and serology) used to isolate and identify N.meningitidis. (This section should contain descriptions and explanations of methods.) [1/2 page]
What are different bacterial disease in plants? Write details about the disease in plants. How the...
What are different bacterial disease in plants? Write details about the disease in plants. How the bacterial disease in plants will be controlled?
class discussions about categories of biochemical molecules
class discussions about categories of biochemical molecules
Interpret Biochemical data for microbial identification. 1. Identify function of positive/negative controls. 2. Use positive and...
Interpret Biochemical data for microbial identification. 1. Identify function of positive/negative controls. 2. Use positive and negative controls to interpret sample test results (e.g test was positive, negative, or can't be determined). 3. Troubleshoot possible problems with biochemicals experiments.
You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your...
You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques which can be used to identify unknown bacteria (you can use any reliable online resources).
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT