Question

Design a study to investigate the sylvatic cycle (wildlife cycle) of a hypothetical mosquito-borne virus. It is known that small mammals such as rodents are the reservoirs. Use critical thinking skills as well as the CDC and the journal Emerging Infectious Diseases to determine how to design this study. Include the hows/whys/wheres of sampling including sample sizes. Include the hows/whys/and types of testing (e.g., PCR or antibody or other, etc.). Address any pitfalls or expected results of your study.

Answer 1

Answer:

• Dengue is a viral disease transmitted by mosquitoes and caused by four viral serotypes belonging to the genus Flavivirus of the Flavivirus family.
• In terms of illness and death, dengue is the most important viral disease transmitted to human by mosquitoes.

Mosquito Sampling:

• Because many arbovirus of medical and veeterinary interest circular in the study area, used variety of sampling methods to collect a wide range of mosquito species.
• Mosquitoes were then sampled by using human landing catches, CDC light trap with or without CO2, and animal bait traps.
• Samples from these tree holes were stored in plastic boxes and flooded in the laboratory for egg hatching.
• Virus isolation were performed on AP61 mosquito cell lines, as described by Digoutte et al. Virus was identified by using immunofluorescence with a specific fixation or neutralization tests.
• The true infection rate estimated number of dengue virus positive mosquitoes per 100 mosquitoes tested
• Nucleic acid detection: RNA is heat labile and therefore specimens for nucleic acid detection must be handled and stored according to the procedures described for virus isolation.
• RT-PCR: In situ RT-PCR offers the ability to detect dengue RNA in paraffin- embedded tissues.
• Extraction and purification of viral RNA from the specimen can be done by traditional liquid phase separation methods but has been gradually replaced by silica based commercial kits that are more reproducible and faster, especially since they can be automated using robotics systems.

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