Question

How do I calculate the corrected area of an unknown in a gas chromatography experiment when you have a standardized set of knowns? Which Rf do you use?

Answer 1

If the Corrected area of an unknown in a gas chromatography experiment have a standardized set of knowns then the known analyte with concentration gradient is added to the sample of unknown as the signal of known whose concentration gradient is added from the set of knowns will help us to determine the concentration in the original sample because signal is proportional to the cocentration of analyte.

A “response factor” or R_f must be calculated for each substance of known with a particular detector. As the response factor is obtained experimentally by analyzing a known quantity of the substance into the GC instrument and measuring the area of the relevant peak.

The experimental conditions (temperature, pressure, carrier gas flow rate) must be identical to those used to analyze the specimen.

The response factor equals the area of the spectral peak divided by the weight or volume of the substance injected.

If proper technique applies then the running of a standard sample before and after running of the specimen, determining a response factor is not necessary.