Question

Explain how gas chromatography differs from liquid chromatography.

Answer 1

Sol:-

The differences between liquid and gas chromatography are :

Gas Chromatography	Liquid Chromatography
1. Can performed only in a column.	1. Can be performed in a column or in a sheet.
2.Can be used in the separation of  gaseous mixtures and volatile compounds.	2. Can be used to separate any soluble compound like proteins, nucleic acids,  amino acids, lipids, natural and artificial polymers, antioxidants and carbohydrates.
3.In this mobile phase is a gas .	3.Here mobile phase is a liquid.
4.Separation is based on the boiling points of solute particles.	4. While here separation is based on interaction between solute and chromatography medium.
5. Performed at higher temperatures so that thermally labile substances get denatured.	5. Performed at room temperature so that heat-sensitive compounds can be safely analyzed.
6.Can use any solvent which vaporizes.	6.Polar solvents like water can be used.
7.The analysis is faster .	7.The analysis is slower.
8. Gives better resolution.	8. Gives lower resolution.
9. it is not very flexible in terms of optimizing separation because Separation is based on the boiling points of the solute particles.	9. it is easy to optimize results because Solute retention is based on the interaction of solutes with the mobile and stationary phases.