Question

Name and briefly describe the three most popular chromatography methods for shotgun proteomics (e.g. size exclusion followed by normal phase)?

Answer 1

This chromatography methods for shotgun proteomics to find the protein in the complex mixture using the following chromatography technique, the technique may be like following

• Bottom-up proteomics
• Mass spectrometry
• Protein mass spectrometry

Bottom-Up Proteomics: This is the most method to identify the protein, this is the method used to analyze the proteolytic digested of protein prior to mass spectroscopy. Determining the protein makeup of a given sample with a cell or tissue.

Mass Spectrometry Technique: The most powerful technology spectrometry-based proteomics, the large-scale analysis of proteins by mass spectrometry, most opted tool.  Many strategies like the incorporation of heavy stable isotopes for differential proteomics have been developed in recent years, includes label-free comparisons with statistical information, with specific information.

Protein mass spectrometry: This is to determine both protein and lipid. Important for the accurate and precise mass determination of protein with many applications.It is used to determine the protein complex, protein submits and global measurement in protein.