Question

Size exclusion chromatography (SEC) is a technique that allows for separation of molecules based on their size. If organic solvents are used to separate polymers, this application is referred to as gel permeation chromatography, and when aqueous solvents are used to separate proteins and soluble macromolecules, this is known as:

a.) Titration

b.) Paper chromatography

c.) P60 chromatography

d.) Silica gel column chromatography

e.) Gel filtration chromatography

Answer 1

Option (e) is correct.

Size exclusion chromatography (SEC) is a technique that allows for separation of molecules based on their size. If organic solvents are used to separate polymers, this application is referred to as gel permeation chromatography, and when aqueous solvents are used to separate proteins and soluble macromolecules, this is known as gel layer chromatography.Gel filteration chromatography is also known as Gel permiation chromatography. Unlike SDS-PAGE which separates the denatured protein based on mass, size exclusion chromatography separates the protein molecules based on its mass and shape.Gel permeation chromatography can be performed using the gravity or attaching the column to a high-pressure pump. The large sample molecules will elute out first, and the smaller molecules will elute out latter. The size of the pores varies with different commercially available beads.