Question

Dr. Singh asks the eye bank for more information about the donor. She finds out that the cornea donor was a previously healthy 30-year-old victim of a motorcycle crash who was on ventilator support for 4 days before his death. The donor’s corneas were harvested 3 days before the transplantations took place and were stored in a buffered medium containing 100 μg/mL gentamicin at 4°C.

How would you determine susceptibility of this P. aeruginosa to gentamicin?

Answer 1

An eye bank is a place where different people donated eyes and cornea transplants for research. This is responsible to harvest and collect the processing corneas to treat and control the cornea. In the different types of countries, the eye bank effectively works to donate the eyes for the needy people. Eye donors are those people whose brain is dead and they are living on a ventilator.
In this case, the doctor asked about more information about the donor because this has been found that the donor was a 30-year-old victim of the crash of a motorcycle who also placed on the ventilator support. The eye is also harvested by the bank before the transplantation takes place. To determine the susceptibility of this P. aeruginosa to gentamicin is by testing the diffusion method. One can check the susceptibility by observing and lack of concentration as well as isolation in the dilution test. Different type of zones which are responsible to enable the gentamicin sulfate that has to be tested by the strain method and inhibition method.Gentamycin is resistant to the strain of P .aeruginosa with the different potential recipient and strains. The surface effect from the protein synthesis the chemically yielded inhibition is observed. They also used to test the susceptibility of gentamycin and other drugs which are sensitive to kill the Pseudomonas.