Question

Sanger sequencing uses dideoxyribonucleotides because

a)They prevent the incorporation of subsequent DNA nucleotides

b)DNA polymerase will not utilize them during replication

c)They work more efficiently at high temperatures

question2

Which of the following is a palindromic sequence capable of being recognized by a restriction enzyme?

Group of answer choices

a)5’- ATGGTA -3’

b.5’- GCTAGC -3’

c.5’- CGCGAG -3’

d.5’- TACGAT -3’

question 3

What is the main difference between benign and malignant tumors?

Group of answer choices

a.Benign tumors can metastasize, while malignant tumors cannot

b.Malignant tumors can metastasize, while benign tumors cannot

c.Both benign and malignant tumors behave identically

question 4

In order to cause phenotypic change at a cellular level, only 1 allele of a tumor supressor gene needs to be inactivated

Group of answer choices

True

False

Benign tumors are caused by DNA mutation, while malignant tumors are not

Malignant tumors are caused by DNA mutation, while benign tumors are not

Answer 1

Question 1

Option a. Dideoxyribonucleotides lack 3'-OH. Therefore, incorporation of these substances inhibit chain elongation, since, additional nucleotides can not be added.

Question 2

Option b. Palindromic sequences are same from 5' to 3' on one strand and 3' to 5' on the complementary strand. So, if we look at the given options, only the sequence in option b will fulfill this requirement, and is therefore a palindromic sequence.

Question 3

Malignant tumors can metastasize, while benign tumors can not. Benign tumors are non cancerous; they are similar to normal cells and are unable to metastasize (ability to spread to distant organs). Malignant tumors, on the other hand, can move apart from primary tumor and spread to distant organs. This feature of malignant tumor makes it different from benign tumor.

Question 4

False. If one allele of tumor suppressor gene is inactivated, the other active allele can still function to suppress tumor formation. Thus, in order to cause phenotypic changes, both alleles of tumor suppressor gene needs to be inactivated.

Malignant tumors are caused by DNA mutation. Benign tumor are like normal cells, they do not have much mutations and therefore, they are non cancerous.