How can de DNA sequencing reveal a protein using Sanger dideoxy DNA

Expert Solution

gladiator answered 1 year ago

explain how Sanger sequencing works

In automated DNA sequencing reaction by the Sanger method, which of the following is NOT required?...

In automated DNA sequencing reaction by the Sanger method, which of the following is NOT required? fluorescently-labeled dideoxynucleotides deoxynucleotides DNA polymerase a DNA primer none of the above (i.e. all the above are required in the reaction)

Draw a workflow of the original Sanger Sequencing and explain how the automated Sanger method is...

Draw a workflow of the original Sanger Sequencing and explain how the automated Sanger method is more efficient.

A random multiple choice question abiut the topic, Dideoxy DNA Sequencing of Sanger?

Sanger sequencing uses dideoxyribonucleotides because a)They prevent the incorporation of subsequent DNA nucleotides b)DNA polymerase will...

Sanger sequencing uses dideoxyribonucleotides because a)They prevent the incorporation of subsequent DNA nucleotides b)DNA polymerase will not utilize them during replication c)They work more efficiently at high temperatures question2 Which of the following is a palindromic sequence capable of being recognized by a restriction enzyme? Group of answer choices a)5’- ATGGTA -3’ b.5’- GCTAGC -3’ c.5’- CGCGAG -3’ d.5’- TACGAT -3’ question 3 What is the main difference between benign and malignant tumors? Group of answer choices a.Benign tumors can...

The DNA polymerases used over many years for Sanger dideoxynucleotide termination sequencing of DNA have had...

The DNA polymerases used over many years for Sanger dideoxynucleotide termination sequencing of DNA have had one common feature: they all were intentionally chosen to lack 3’à 5’ exonuclease activity, the proofreading function that many DNA polymerases carry to maintain high fidelity. Why would having proofreading exonuclease activity within the polymerase be detrimental to the Sanger sequencing technique? If you were to substitute a polymerase with proofreading capability, how might that affect the distribution of the bands of DNA produced...

Why does nextgen sequencing work more effectively than Sanger sequencing when working with ancient DNA samples?...

Why does nextgen sequencing work more effectively than Sanger sequencing when working with ancient DNA samples? What features of sequences can be used to separate sequences generated from ancient samples vs modern contaminants?

sanger sequencing, and how base identity (A,T,C,G) is detected using fluorescence. Can you explain what it...

sanger sequencing, and how base identity (A,T,C,G) is detected using fluorescence. Can you explain what it means that the DNA is labeled with a fluorescent tag, and where that tag is located?

genetics chapter 10 How does Sanger sequencing work?

: Sanger sequencing is the method of dideoxy sequencing that we use in Bio 2450. It...

: Sanger sequencing is the method of dideoxy sequencing that we use in Bio 2450. It is a modified PCR reaction that has the dNTPS replaced with a mixture of dNTPs:ddNTPs. In this section, go into detail on how the addition of ddNTP alters the produced PCR fragments and describe how we ultimately get the sequence.

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