A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood...

A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood enzyme reactions. It has been studied extensively and we know the two critical side chains in the active site of TIM that catalyze the isomerization reaction. Write the mechanism of TIM showing the two catalytic side chains.

B) In the TIM reaction, the histidine residue starts off neutral, not positively charged (as one might expect since it donates a proton to the O-group). Explain why it makes energetic sense for the histidine not to start off positively charged.

Solutions

Expert Solution

gladiator answered 1 year ago

Next > < Previous

Related Solutions

Both glyceraldehyde 3-phosphate and dihydroxyacetone phosphate are substrates for the enzyme triose phosphate isomerase. In a...

Both glyceraldehyde 3-phosphate and dihydroxyacetone phosphate are substrates for the enzyme triose phosphate isomerase. In a study by Knowles et al the kM for glyceraldehyde 3-phosphate was determined to be 0.47mM with a Vmax of about 410 uM/min. Whereas, for the substrate dihydroxyacetone phosphate the kM was determined to be 0.97 mM and the Vmax was about 41 uM/min. (Biochem J 129 (2) 1972:301-310). Triose phosphate isomerase has a relatively weak affinity for _______ A. glyceraldehyde 3-phosphate because the kM...

Fructose-1,6-bisphosphate (FBP) <=====> glyceraldehyde-3-phosphate (GAP) + dihydroxyacetone phosphate (DHAP) The standard free energy change for this...

Fructose-1,6-bisphosphate (FBP) <=====> glyceraldehyde-3-phosphate (GAP) + dihydroxyacetone phosphate (DHAP) The standard free energy change for this reaction is +22.8 kJ/mol. Under actual conditions in the cell the concentrations of GAP,DHAP,and FBP are 0.5 mM, 0.3 mM, and 6.0 mM respectively. Given this information calculate the actual free energy change for this reaction in the cell at a temperature of 37 oC. Provide your answer (just the numerical part) in kJ/mole to two signicant figures

In the glycolytic pathway, the enzyme triose phosphate isomerase catalyzes the conversion of of dihydroxyacetone phosphate...

In the glycolytic pathway, the enzyme triose phosphate isomerase catalyzes the conversion of of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, which is then immediately utilized by glyceraldehyde-3-phosphate hydrogenase to continue through glycolysis. In an experimental cell culture model, inhibition of triose phosphate isomerase leads to cell death in anaerobic conditions. These cells survive, however under aerobic conditions. Please explain these findings. In your answer consider the energy production in aerobic vs. anaerobic metabolism.

One molecule of glyceraldehyde-3-phosphate is fed into glycolysis. Which and how many electron carrier molecule(s) are...

One molecule of glyceraldehyde-3-phosphate is fed into glycolysis. Which and how many electron carrier molecule(s) are made during (A) the glycolytic breakdown of this molecule, during (B) acetyl-CoA production and during (C) the citric acid cycle? (D) How many H+ are translocated across the mitochrondrial membrane due to the re-oxidation of these electron carriers? Write the numbers into the respective boxes. This question is very important for me to understand. Please only respond if you are answering all parts and...

1.) a key reaction in glycolysis is the isomerization of dihydroxyacetone into gylceraldehyde-3-phosphate. dhap<> G-3-P Delta...

1.) a key reaction in glycolysis is the isomerization of dihydroxyacetone into gylceraldehyde-3-phosphate. dhap<> G-3-P Delta G = 7.5 kj/mol a.) whis is favored in this equilibrium, DHAP OR G-3-P? B.) Calculate the equilibrium constant for the reaction at 25 degrees C. C.) in the cell depletion of G-3-P makes the reaction proceed. what will delta G be if the temperature concentration of G-3-P is always kept 1/100 of the concentration of DHAP at a temp of 37 degrees C?

1. What is the effect that glyceraldehyde 3 phosphate dehydrogenase has on cells? 2. What would...

1. What is the effect that glyceraldehyde 3 phosphate dehydrogenase has on cells? 2. What would happen in the absence of Pol1, if the the primers are not replaced by DNA and if Pol 3 was absent and no DNA synthesis occured? 3. How does the phase where Glucose forms glucose 6 phosphate differ from the phase where PGA forms pyruvate in terms of reaction coupling to overcome thermodynamic hurdles for glycolysis to occur ***

QUESTION 39 The molecule that traps the sun's energy is   ATP.  chloroplast.  chlorophyll.  glyceraldehyde-3-phosphate &nbsp

QUESTION 39 The molecule that traps the sun's energy is   ATP.  chloroplast.  chlorophyll.  glyceraldehyde-3-phosphate QUESTION 42 Deleterious mutations increase the fitness of the organism. True False QUESTION 47 The light-independent reactions of photosynthesis (Calvin cycle) take place in the   cytoplasm.  grana.  thylakoids.  stroma. QUESTION 48 Cellular respiration is endergonic. True False QUESTION 49 The ATP and NADPH produced in the light-dependent reaction stage   are the end products of photosynthesis.  become reactants for cellular respiration.  are waste products that the...

1) write the balanced net reaction of the conversion of glyceraldehyde 3-phosphate into lactate 2)If all...

1) write the balanced net reaction of the conversion of glyceraldehyde 3-phosphate into lactate 2)If all the glucose were metabolized through that 2,3-BPG shunt into lactate, what should be the net reaction for the conversion of glucose to lactate?

In order to produce three glyceraldehyde-3-phosphate molecules that the plant could use to make other organic...

In order to produce three glyceraldehyde-3-phosphate molecules that the plant could use to make other organic molecules, how many CO2 molecules would have to enter the Calvin Cycle? 3 6 9 12 Genes A and B are on the same chromosome. If a person has one chromosome with the alleles AB and a second chromosome with the alleles ab, how could they produce a gamete with a chromosome containing the alleles Ab or aB? independent assortment random fertilization crossing over...

1. Explain how to convert positive integers from base 3 to base 10 and vice versa....

1. Explain how to convert positive integers from base 3 to base 10 and vice versa. Include examples. 2. Explain how to convert fractions from base 3 to base 10 and vice versa. Include examples.

Subjects