A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood...

A)Triosephosphate isomerase(TIM)converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3-phosphate (GAP) and vice-versa. This is one of the best-understood enzyme reactions. It has been studied extensively and we know the two critical side chains in the active site of TIM that catalyze the isomerization reaction. Write the mechanism of TIM showing the two catalytic side chains.

B) In the TIM reaction, the histidine residue starts off neutral, not positively charged (as one might expect since it donates a proton to the O-group). Explain why it makes energetic sense for the histidine not to start off positively charged.

Expert Solution

gladiator answered 2 years ago

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