Question

In: Biology

2. Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is...

2. Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cell‑free translation system or in any other such system Further analysis indicates that this virus replication is inhibited if infected cells are incubated with protease inhibitor such as trypsin inhibitor. Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells.

Solutions

Expert Solution


Related Solutions

Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable...
Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cellfree translation system or in any other such system Further analysis indicates that this virus replication is inhibited if infected cells are incubated with protease inhibitor such as trypsin inhibitor. Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells.
1. Suppose that you isolated an enveloped icosahedral RNA virus. Purified RNA from this virus is...
1. Suppose that you isolated an enveloped icosahedral RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cell‑free translation system or in any other such system. Further analysis indicates that this virus replication is not inhibited by actinomycin D, a strong inhibitor of DNA-dependent RNA polymerase. Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells. Please give more information that ties in replication...
Why do you suppose an animal virus grown in (and then isolated from) tissue culture might...
Why do you suppose an animal virus grown in (and then isolated from) tissue culture might be more susceptible to inactivation by antibody than a similar virus propagated (and then isolated from) in an animal? Assume you do the assay by isolating the viruses grown in these two different environments from antibody and then seek to compare in a controlled environment, which virus particle is inactivated more readily by the presence of appropriate antibody.
Hepatitis B Virus: 1. Describe the viral architecture 2. Nucleic acid composition 3. Enveloped or non-enveloped?...
Hepatitis B Virus: 1. Describe the viral architecture 2. Nucleic acid composition 3. Enveloped or non-enveloped? 4. Host(s)? How does the virus gain entrance to host? 5. Mode of transmission(s)? 6. Organ System(s) affected? 7. Symptoms of viral disease. 8. Treatments? 9. Acute or persistent infection and why?
You have purified genomic RNA from the following viruses: Zika, and murine leukemia viruses. You performed...
You have purified genomic RNA from the following viruses: Zika, and murine leukemia viruses. You performed an experiment where susceptible, permissive cells were separately transfected with one pure molecule of viral RNA. Which RNA will lead to a productive viral infection? Discuss the reasons why one RNA succeeds and the other fails. Be clear and convincing
Suppose a student carrying a flu virus returns to an isolated college campus of 1000 students.
Suppose a student carrying a flu virus returns to an isolated college campus of 1000 students. Determine a differential equation governing the number of students x(t) who have contracted the flu if the rate at which the disease spreads is proportional to the number of interactions between the number of students with the flu and the number of students who have not yet been exposed to it
BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)is an enveloped, (+)-sense, single-strandRNA virus that causes coronavirus disease...
BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)is an enveloped, (+)-sense, single-strandRNA virus that causes coronavirus disease 2019(COVID-19). The coronavirus family is named for the crown-like spikes on the outer surface of the virus and includes viruses that infect many species of animals, including camels, cattle, bats, and cats. The SARS-CoV-2 virus appears to have “spilled”out ofbats,its primaryanimal host,and into humansin December of 2019 in Wuhan, China. COVID-19 symptoms appear 2-14 days after exposure and include fever, dry cough, shortness of...
Various experiments have shown that the SARS CoV-2 is an RNA virus that enter the human...
Various experiments have shown that the SARS CoV-2 is an RNA virus that enter the human cells through a transmembrane receptor called angiotensin converting enzyme 2 (ACE2). Polymerase chain reaction (PCR) and genome sequencing have been cardinal in both diagnosis and research into the COVID-19 pandemic. Currently, DNA sequences of both SARS CoV-2 virus and susceptible human hosts are available. As a student of molecular biology and biotechnology, use the above information to answer the following questions. 9.In the study...
Various experiments have shown that the SARS CoV-2 is an RNA virus that enter the human...
Various experiments have shown that the SARS CoV-2 is an RNA virus that enter the human cells through a transmembrane receptor called angiotensin converting enzyme 2 (ACE2). Polymerase chain reaction (PCR) and genome sequencing have been cardinal in both diagnosis and research into the COVID-19 pandemic. Currently, DNA sequences of both SARS CoV-2 virus and susceptible human hosts are available. As a student of molecular biology and biotechnology, use the above information to answer the following questions. 6. Given the...
What do you understand from helical CT? Discuss. What are the advantages of helical CT over...
What do you understand from helical CT? Discuss. What are the advantages of helical CT over the step and shoot sequential CT? What is the purpose of antiscatter grid in a CT instrument? Discuss its use in CT. What is filter back projection and how is it used in CT?
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT