Sketch a general Lineweaver-Burke plot that illustrates the kinetic behavior of a non-allosteric enzyme. Identify on...

Sketch a general Lineweaver-Burke plot that illustrates the kinetic behavior of a non-allosteric enzyme. Identify on your plot the features that allow one to determine Vmax and KM for the enzyme.

How would one use a Lineweaver-Burke plat of enzyme kinetic data to identify an inhibitor's mode of action?

Expert Solution

queen_honey_blossom answered 1 year ago

use of a Lineweaver-Burke plot to determine enzymatick parameters

Draw a double reciprocal plot for a typical Michaelis Menton enzyme and an allosteric enzyme that...

Draw a double reciprocal plot for a typical Michaelis Menton enzyme and an allosteric enzyme that has the same Vmax and Km value. Also raw a double reciprocal plot for for the allosteric enzyme in the absence and presence of the allosteric inhibitor.

I need to calculate the turnover rate of an enzyme using a lineweaver-burk plot. Is there...

I need to calculate the turnover rate of an enzyme using a lineweaver-burk plot. Is there a specific equation that should be used to calculate Kcat? I have been unable to find a straightforward answer on how to calculate Kcat!

Why is a V vs S plot often inadequate for determining enzyme kinetic constants, and how...

Why is a V vs S plot often inadequate for determining enzyme kinetic constants, and how do various alternate plotting methods allow these values to be determined more adequately? Give examples of alternate methods of kinetic analysis and their utility. The V vs S plot is inadequate because it makes the assumption that it’s only for a single subunit and substrate. The line on the graph usually represents a line that best fits the data and are not completely accurate....

Identify and describe the steps of the scientific method. Identify general trends in a data plot....

Identify and describe the steps of the scientific method. Identify general trends in a data plot. Convert between SI and American units, as well as within units of the same system (i.e., from feet to miles). Use kinetic/mathmatical equations to find acceleration, velocity, position, or time. What sre Newton’s three laws of motion. How to Add vectors, including vectors that represent forces and velocity. Explain the difference between weight and mass. Explain the concept of conservation of linear and angular...

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