Question

Briefly discuss any source of errors in the HPLC Lab ''Liquid Chromatographic Analysis of Analgesic Powders''

Answer 1

High Performance Liquid Chromatography (HPLC) has resulted from the application of gas chromatography instrumental techniques to classical liquid chromatography. In classical liquid chromatography (column chromatography), a solid material (stationary phase) is packed into a column and the mixture to be separated is introduced onto the top of the column. An eluting liquid (mobile phase) is washed through the column. If a component in the mixture is strongly attracted to the stationary phase, it will be retarded on the column and will be separated from components that are more weakly attracted to the stationary phase. There are four main mechanisms by which separation occurs. In adsorption chromatography, the sample components are selectively adsorbed onto the surface of the solid stationary phase. In liquid-liquid chromatography the stationary phase is a liquid coated onto or chemically bonded to a finely divided inert solid support. Separation occurs due to partitioning of the sample components between the mobile and the stationary phases. In normal phase chromatography the mobile phase is nonpolar and the stationary phase is polar. In reverse phase chromatography, the mobile phase is polar and the stationary phase is nonpolar. This experiment will utilize reverse phase chromatography with an octadecyl (C-18) column and a polar mobile phase consisting of methanol, acetonitrile, and water