Can you please explain what the roles of LacY, LacZ, Operators, IPTG, and Lac Repressors in lactose...

Can you please explain what the roles of LacY, LacZ, Operators, IPTG, and Lac Repressors in lactose metabolism

Expert Solution

Ans. Lac Y - codes for Lactose permease that help in transfer of lactose inside the cell.

Lac Z - codes for Beta-galactosidase digest lactose into glucose and galactose.

Operator- operator controls transcription of structural genes It lies adjacent to structural genes.When operator is turned off by repressor, transcription of structural genes don't occur While when operator is turned on by inducer, repressor is removed and transcription of structural genes occur.

IPTG- Isopropyl β- d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose. So IPTG have high affinity for repressor and now repressor not bind to operator and transcription starts.

Lac repressor- lac repressor is coded by lac I gene. This repressor is a dimer and have DNA binding domain. The repressor binds to operator in tetramer form and DNA get folded. So transcription is inhibited.

gladiator answered 2 years ago

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