Question

What property allowed us to observe analgesics under the UV light?

Answer 1

Analgesics are pain relieving drugs. They have specific functional group which react with adsorbent and under uv light it will give colourd bend, by TLC method.

Thin Layer Chromatography can be used for small-scale preparative separations, employing thicker layers of adsorbent and applying the sample as a band instead of individual spots. The chromatogram can be developed, and the edges of the plate treated with visualizing agents to locate the bands of interest. The position of the bands on the developed TLC plate can alternately be located under ultra-violet light. Once the bands are located, they are scraped from the plates and the material leached from the adsorbent using an appropriate solvent. The dissolved components of the bands can then be analyzed by other techniques, including UV spectroscopy.