In: Chemistry
A developed TLC plate was examined under UV light. Was it a necessary step if both ferrocene and acetylferrocene are visible without a visualization method? Why or why not?
I need at least a paragraph response to this question!
Thin layer chromatography (TLC) is a useful technique commonly used for the qualitative determination of the purity of samples as well as the relative polarities and separation of the components present in a mixture. In the visualisation part, we should look at our TLC plate under ultraviolet light. The dye present in the TLC plate will glow green under UV light. Many compounds absorb UV light, leaving dark spots on the plate. Basically visualisation method is required for colourless compounds. Ferrocene and acetylferrocene are strongly coloured, so we will be able to see the spots without any visualisation method depending on the fact that the colour should be prominent on TLC plate.