So in our Ochem TLC experiment. We prepared some of our solid samples dissolving them in...

So in our Ochem TLC experiment. We prepared some of our solid samples dissolving them in ethanol, and we prepared our liquid samples by mixxing them with dichloromethane. My question is...

Why doesn’t it matter that some of the samples are prepared using ethanol as a solvent and other samples are prepared using dichloromethane as a solvent? [Hint: What happens to this solvent after you deposit a spot of solution to the TLC plate?]

Expert Solution

choosing solvent system depends on the compound which you would like to seperate.Depending on the polarity of the components in the compound, different components travel different distances up the plate. considering the silica plates, more polar components will stick to polar silica gel and travel short distances, while non polar components diffuse into the solvent and travel long distances.

After depositing the spot on the plate, the place should be placed upright, slightly inclined to the wall of the container.By capillary action the solvent immediately strat rising up the TLC. as soon as it comes into contact with the spots it will cause the components to move based on their polaity.

queen_honey_blossom answered 2 years ago

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