DNA may be synthesized by primer elongation methods such as PCR, and if radioactive atoms are incorporated into the newly made DNA

DNA may be synthesized by primer elongation methods such as PCR, and if radioactive atoms are incorporated into the newly made DNA, it may be used as a hybridization probe in methods like Southern blotting. To incorporate radiolabeled nucleotides into newly synthesized DNA, researchers may use 32P-labeled dATP, in which one of the P atom is radioactive32P. Which of the 3 phosphate groups (alpha, beta, or gamma) should be labeled, and why?

Expert Solution

queen_honey_blossom answered 2 years ago

Short segments of newly synthesized DNA are joined into a continuous strand by _____.

Part A Short segments of newly synthesized DNA are joined into a continuous strand by _____. DNA polymerase single-strand binding protein ligase helicase primase Part B After DNA replication is completed, _____. each of the four DNA strands consists of some old strand parts and some new strand parts one DNA double helix consists of two old strands and one DNA double helix consists of two new strands there are four double helices each new DNA double helix consists of two...

Why does DNA sequencing reaction use one primer and PCR use two primers?

Describe how newly synthesized fatty acids are incorporated into triacylglycerols. (please type the answer, no handwriting,...

Describe how newly synthesized fatty acids are incorporated into triacylglycerols. (please type the answer, no handwriting, and maybe you can draw a diagram to help explain it !)

1. What is a primer? What are they made out of during DNA replication? Why does...

1. What is a primer? What are they made out of during DNA replication? Why does the process of DNA replication require the use of a primer? 2. When an agarose gel is set up, the DNA samples are put at one end of the gel and the pieces of DNA migrate toward the other end. a. At what end are the DNA samples put? (1 point) b. Toward what end do the DNA samples migrate? (1 point) c. What...

Breifly describe two methods one might use to find the molar mass of a newly synthesized...

Breifly describe two methods one might use to find the molar mass of a newly synthesized gas for which a molecular formula was not known.

DNA sequence may be used to identify different species. Assuming that you can use PCR to...

DNA sequence may be used to identify different species. Assuming that you can use PCR to generate products for sequencing, describe in detail an experiment that will allow you to determine which strain of Salmonella has caused the more recent outbreak.

Describe how CI-M6PR deliveries newly-synthesized enzymes to the lysosome? You may use diagrams to explain or...

Describe how CI-M6PR deliveries newly-synthesized enzymes to the lysosome? You may use diagrams to explain or enhance your answer. What’s part of the responsible for the mechanism underlying I-cell disease.

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