DNA may be synthesized by primer elongation methods such as PCR, and if radioactive atoms are incorporated into the newly made DNA

DNA may be synthesized by primer elongation methods such as PCR, and if radioactive atoms are incorporated into the newly made DNA, it may be used as a hybridization probe in methods like Southern blotting. To incorporate radiolabeled nucleotides into newly synthesized DNA, researchers may use 32P-labeled dATP, in which one of the P atom is radioactive32P. Which of the 3 phosphate groups (alpha, beta, or gamma) should be labeled, and why?

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queen_honey_blossom answered 1 year ago

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