could you have achieved a better separation on your TLC plate between aspartic acid and phenylalanine?

Expert Solution

Yes. With mobile phase composition butanol–glacial acetic acid, in the ratio of 70:30. R_F values could be 0.0 and 0.80 for aspartic acid (Asp - acidic amino acid - polar) and phenylalanine (Phe - Non-Polar) respectively. We know that polar substances tend to move slowly in TLC compare to non-polar substances. This rule is universal.

Please look at the page number 2 and Table Number 3 at below pdf published journal.

https://www.researchgate.net/file.PostFileLoader.html?id=52e627aed039b1094f8b4631&assetKey=AS%3A272426133327881%401441962859703

In another mobile phase n-butanol:acetic acid:water 3:1:1(by volume) mixture followed by ninhydrin stain the R_F values are found to be Asp - 0.24 and Phe - 0.62 respectively. Please have a look at Reach Devices (Made in USA)

http://www.reachdevices.com/TLC_aminoacids.html

Thank You so much!

queen_honey_blossom answered 2 years ago

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