The plate has 259 colonies, with a sample volume of 10^(-6) mL. What was the original...

The plate has 259 colonies, with a sample volume of 10^(-6) mL. What was the original concentration in the sample? How many colonies should be on the plate inoculated with a sample volume of 10^(-7) mL using the same sample?

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Expert Solution

plate has = 259 colonies

volume = 10^-6 mL

original concentration = 259 / 10^-6

original concentration = 2.59 x 10^8 CFU / mL

number of colonies = concentration x volume

= 2.59 x 10^8 x 10^-7

= 25.9

number of colonies = 26 colonies

queen_honey_blossom answered 1 year ago

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