In: Chemistry
The plate has 259 colonies, with a sample volume of 10^(-6) mL. What was the original concentration in the sample? How many colonies should be on the plate inoculated with a sample volume of 10^(-7) mL using the same sample?
plate has = 259 colonies
volume = 10^-6 mL
original concentration = 259 / 10^-6
original concentration = 2.59 x 10^8 CFU / mL
2)
number of colonies = concentration x volume
= 2.59 x 10^8 x 10^-7
= 25.9
number of colonies = 26 colonies