Question

In: Chemistry

The plate has 259 colonies, with a sample volume of 10^(-6) mL. What was the original...

The plate has 259 colonies, with a sample volume of 10^(-6) mL. What was the original concentration in the sample? How many colonies should be on the plate inoculated with a sample volume of 10^(-7) mL using the same sample?

Solutions

Expert Solution

plate has = 259 colonies

volume = 10^-6 mL

original concentration = 259 / 10^-6

original concentration = 2.59 x 10^8 CFU / mL

2)

number of colonies = concentration x volume

                               = 2.59 x 10^8 x 10^-7

                               = 25.9

number of colonies   = 26 colonies


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