An enzyme catalyzes the reaction S → P. The table shows how the
reaction rate (v) of the enzyme varies with the substrate
concentration [S].
0,0500
0,04545
0,0250
0,03448
0,0167
0,02778
0,0125
0,02326
0,0100
0,02000
a) Create a Lineweaver-Burk plot of the data given in the table
above.
b) Calculate Km, Vm, and Vm / Kmfor the enzyme.
c) Also calculate kcat and kcat / Km (the specificity constant)
when given that
[E] = 10 nM.d) How saturated will an...