The native and denatured forms of a protein are generally in equilibrium as follows: &

The native and denatured forms of a protein are generally in equilibrium as follows:

Protein (denatured) <--> Protein (native)

For a certain solution of the protein ribonuclease A, in which the total protein concentration is 2.00 x 10^-3 M, the concentrations of the denatured and native proteins at both 25.0°C and 37.0°C are given in the following table.

Temperature (C°)	[Ribonuclease A (denatured)] (M)	[Ribonuclease A (native)] (M)
25.0	5.10 x 10^-6	2.00 x 10^-3
37.0	2.80 x 10^-4	1.70 x 10^-3

a. Determine ∆G°, ∆H° and DS° for the folding reaction at 27.0° C assuming that it is independent of temperature.

b. What is the denaturation temperature of ribonuclease A under standard state conditions? (This is the temperature at which the K_eq = 1.00)

please answer b- I know the answer to a, but have no idea how to approach b

Expert Solution

b) I shall need the calculations for part (a). I will tell you how to do it though.

You have said that you got part (a) correct which means that you have found out numeric values for ΔG⁰, ΔH⁰ and ΔS⁰.

You need to assume that ΔH⁰ and ΔS⁰ are independent of temperature, i.e, whatever numeric values you have for ΔH⁰ and ΔS⁰ will stay constant throughout.

You are given that K_eq = 1.000; find out ΔG⁰ using the relation ΔG⁰ - -R*T*ln K_eq where T is the desired temperature.

We know that ln (1.000) = 0 and hence, ΔG⁰ = 0.

Next use the relation

ΔG⁰ = ΔH⁰ – T*ΔS⁰ = 0

====> T = ΔH⁰/ΔS⁰

Simply divide the value of ΔH⁰ by the value of ΔS⁰ obtained in part (a) and you will get T; hope this helps.

queen_honey_blossom answered 2 years ago

Functional assays vs. protein density: what happens to each if protein is denatured

explain the mechanism how the double distilled water may denatured the tertiary structure of protein ?

Develop a hypothesis to explain why the protein is folded in its native state

A newly synthesized protein generally folds up into a __________________ conformation.

A newly synthesized protein generally folds up into a __________________ conformation. All the information required to determine a protein’s conformation is contained in its amino acid __________________. On being heated, a protein molecule will become __________________ as a result of breakage of __________________ bonds. On removal of urea, an unfolded protein can become __________________. The final folded conformation adopted by a protein is that of __________________ energy.Words: denatured composition noncovalent irreversible stable reversible highest covalent renatured lowest unstable sequence

The helix that forms in a protein chain as a result of hydrogen bonds and other...

The helix that forms in a protein chain as a result of hydrogen bonds and other weak forces is an example of the Multiple Choice non-linear structure of protein. secondary structure of protein. primary structure of protein. tertiary structure of protein.

a) The term ‘amyloid’: Was coined by the German physician, Alois Alzheimer Generally refers to protein...

a) The term ‘amyloid’: Was coined by the German physician, Alois Alzheimer Generally refers to protein aggregates of any form Protein aggregates that stain similarly to the polysaccharide, cellulose when exposed to iodine. None of the above. b) Which of the following is true about Tay Sachs disease? Choose all that apply. It results from an abnormal accumulation of the enzyme, HexA in lysosomes. It results in the abnormal accumulation of GM2 ganglioside inside lysosomes. It is caused by a...

Since insurance contracts are generally standard forms with manydetailed provisions drawn up by the insurer,...

Since insurance contracts are generally standard forms with many detailed provisions drawn up by the insurer, they are considered to be:A. contracts of adhesion.B. unenforceable if the insured cannot negotiate terms.C. insurable interests.D. contrary to the indemnity principle.

Elemental carbon usually exists in one of two forms: graphite or diamond. It is generally believed...

Elemental carbon usually exists in one of two forms: graphite or diamond. It is generally believed that diamonds last forever. The table shows the standard enthalpy of formation (ΔH∘f) and the standard molar entropy (S∘) values for diamond and graphite. Substance ΔH∘f (kJ/mol) S∘ (J/mol⋅K) Cgraphite 0 5.740 Cdiamond 1.897 2.38 Part A What is the standard Gibbs free energy for the transformation of diamond to graphite at 298 K? Cdiamond→Cgraphite Express your answer to three significant figures and include...

Compared with the sole proprietorship and partnership forms of business organization, the corporate form generally faces...

Compared with the sole proprietorship and partnership forms of business organization, the corporate form generally faces with ________ difficulty in raising capital and transferring ownership, ________ taxation, and _______ agency problems. a. Less; Less; More b. Less; More; Less c. Less; More; More d. More; More; Less e. More; Less; More

A study is done to examine the retention of two forms of protein (P1, P2) given...

A study is done to examine the retention of two forms of protein (P1, P2) given to rabbits at each of two concentrations (10.0 and 2.0 milimoles). Twelve rabbits were randomly divided into four groups, one group for treatment combination. There were three rabbits assigned to each combination. The following sums of squares are computed. Source df SS MS F Protein 18.8 Concentration 1 Interaction 2.1 Error 133.3 Total 11 406.0 State the hypothesis to be tested first. Fill in...

Question