Explain the termination of Polymerase I, II, and III.
Explain the termination of Polymerase I, II, and III.
Solutions
Expert Solution
A polymerase is an enzyme that synthesize long chain of
polymers or nucleic acids. DNA polymerase are used to assemble DNA
molecules and RNA polymerase are used to assemble RNA
molecules.
The main function of DNA polymerase is to synthesize DNA from
deoxyribonucleotides.The function of DNA polymerase is to
replicate, proof read and repair DNA.DNA polymerase I, and DNA
polymerase III are the main DNA polymerase involved in DNA
replication.DNA polymerase II is responsible for the association
and dissociation of the DNA strand to the catalytic subunit.
RNA polymerase is an enzyme that is responsible for copying a
DNA sequence into an RNA sequence during the process of
transcription.
RNA polymerase I transcribes rRNA genes, RNA polymerase II
transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA
polymerase III transcribes tRNA and 5S rRNA genes.
What would happen if we use Polymerase I instead of Polymerase
III for DNA replication in a PCR? If we used an enzyme with low
fidelity, how would the DNA product compare to the original
template?
1. Why does E. coli need both DNA polymerase III and DNA
polymerase I?
a. The DNA replication is bidirectional; one polymerase is used
for each direction.
b. Each polymerase is specific for only one strand of DNA. DNA
polymerase III acts only on the leading strand, and DNA polymerase
I acts only on the lagging strand.
c. Only DNA polymerase I has proofreading ability.
d. DNA polymerase III lacks the 5' → 3' exonuclease activity
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2014 I 31 2017 I 69 II 24 II 54 III 23 III 46 IV 16 IV 32 2015 I
42 2018 I 82 II 35 II 66 III 30 III 51 IV 23 IV 38 2016 I 53 2019 I
91 II 45 II 72 III 39 III 59 IV 27 IV 41 Create a multiple
regression equation incorporating both a trend (t=0 in 2013: IV)
and dummy variables for the quarters. Let the first quarter
represent the reference...
2014 I 31 2017 I 69
II 24 II 54
III 23 III 46
IV 16 IV 32
2015 I 42 2018 I 82
II 35 II 66
III 30 III 51
IV 23 IV 38
2016 I 53 2019 I 91
II 45 II 72
III 39 III 59
IV 27 IV 41
Create a multiple regression equation incorporating both
a trend (t=0 in 2013: IV) and dummy variables for the quarters. Let
the first quarter represent the reference...
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