These questions refer paper of "Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast(Deutschbauer et al.,...

These questions refer paper of "Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast(Deutschbauer et al., 2005)"

The yeast genome contains a single gene for ribosomal protein 24 of the 40S ribosome (gene is designated RPS24); it is located on chromosome IV. How many copies of this gene are present in:

A haploid yeast cell?

A tetraploid yeast cell?

A diploid strain that is monosomic for chromosome IV?

A homozygous deletion strain that includes the entire RPS24 locus?

A haploid strain in which the RPS24 gene contains a small deletion?

Expert Solution

Diploid condition: when a cell has pair of identical chromosomes.

Haploid : when a cell has single chromosome of one type.

Tetraploid : when a cell has four copy of an identical chromosome.

So, let to know that a gene RPS24 which is given, resides on chromosome IV. This gene has single copy and found only on chromosome IV.

A haploid yeast cell has a half set of chromosomes or single chromosome of each type. Also chromosome IV will be single thus it has only one gene RPS24.

A tetraploid cell has four identical chromosome of each type and also it has four chromosome IV. Thus this type of cell has four RPS24 gene because each of the chromosome has a gene.

A diploid cell has two set of identical chromosomes. Thus it also has two chromosomes of each type but since it is monosomy for chromosome IV so it has only single chromosome IV and also has a single copy of gene RPS24.

Homozygous deletion of entire locus of RPS24 results in loss of the gene from all the sites possible. So it does not have any copy of enzyme RPS24.

A haploid strain has single set of each of the chromosome. When a small segment deletion will takes place in the gene, it results in failure of synthesis of the gene RPS24, so it also does not has any copy of the gene RPS24.

gladiator answered 1 year ago

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