Question

In: Chemistry

#6... 4. How would you make 500 ml of 5 M NaCl? 5. How would you...

#6...

4. How would you make 500 ml of 5 M NaCl?

5. How would you make 1 liter of 2 M sodium citrate?

6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 M sodium citrate, using the solutions you made in Q4 and Q5 above?

Expert Solution

4.How would you make 500 ml of 5 M NaCl?

Answer: Molarity = (weight of Solute/Mol.weight) (1/volume in L)

5M = (weight of NaCl/ 58.44 g/mol )(1/0.5L)

Weight of NaCL required = 5 M x 0.5 L x58.44 = 146.1 g

Therefore, we need to weigh 146.1 g of NaCl and dissolve into 500 mL solution to make our required solution(500mL of 5M NaCl).

5. How would you make 1 liter of 2 M sodium citrate?

Answer : Molarity = (weight of Solute/Mol.weight) (1/volume in L)

2 M = (weight of Sodium Citrate)/ 258

Weight of Sodium Citrate= 516 g

Therefore, we need to weigh 516 g of sodium citrate and dissolve it into 1 L water. This will give us the 1L of 2M sodium citrate solution.

6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 M sodium citrate, using the solutions you made in Q4 and Q5 above?

20X SSC solution contains 3M NaCl and 0.3 M sodium citrate.

We need to find out how much volume of 2M sodium citrate and 5M of NaCl is required.

Let us find out for NaCl:

Initial volume V1 =?

Initial concentration M1 = 5M

Final Volume V2 = 1L

Final Concentration M2 = 3M

M1V1 = M2V2

V1= M2V2/M1 = 3M x 1L /5M = 0.6 L

For sodium citrate:

Initial volume V1 =?

Initial concentration M1 = 2M

Final Volume V2 = 1L

Final Concentration M2 = 0.3M

M1V1 = M2V2

V1= M2V2/M1 = 0.3M x 1L /2M = 0.15 L

Therefore, we need to mix 600mL of 5M NaCl and 150 mL of sodium citrate solution and make up the final volume to 1L(i.e, 250 mL of water).

queen_honey_blossom answered 2 years ago

If you have 1 M Tris-HCl solution NaCl 100% Tween How would you make 500 mL...

If you have 1 M Tris-HCl solution NaCl 100% Tween How would you make 500 mL of 0.5 M NaCl, 0.1% Tween, and 100 mM Tris-HCl? i suck at buffer questions.... please help !!!

5. You have a dialysis bag containing 15-mL protein solution in 0.20 M NaCl. a. Would...

5. You have a dialysis bag containing 15-mL protein solution in 0.20 M NaCl. a. Would it be more efficient to dialyse this solution against i)one 2 L volume of buffer until equilibrium is reached, or to ii)dialyze the solution against one L buffer until equilibrium is reached, the remove the bag and dialyze against another one L of buffer til equilibrium is reached. Assume there is no volume change in the dialysis bag with dialysis. (1 pt) {type answer...

You have a 5 mL sample of a protein in 0.5 M NaCl. You place the...

You have a 5 mL sample of a protein in 0.5 M NaCl. You place the protein/salt sample inside dialysis tubing (see below) and place the bag in a large beaker of distilled water. If your goal is to remove as much NaCl from the sample as possible, which would be more effective: (1) placing the dialysis bag in 4 L of distilled water for 12 h, or (2) placing the bag in 1 L of distilled water for 6...

4. 62.4 mL of 0.39 M NaCl solution was added to 26.8 mL of 058 M...

4. 62.4 mL of 0.39 M NaCl solution was added to 26.8 mL of 058 M Pb(NO3)2 solution. a. Write the full balanced reaction, the molecular ionic equation and the net ionic equation. b. What is the precipitate? c. How many grams of the precipitate can be made

1) a) How would you make 600 ml of a 0.25 M ammonia and 0.20 M...

1) a) How would you make 600 ml of a 0.25 M ammonia and 0.20 M ammonium nitrate from a bottle of 6.0 M ammonia (aq) and a bottle of solid ammonium nitrate. grams of ammonium nitrate? mL of ammonia? mL of H2O? b) Calculate pH (Kb = 1.8 x 10^-5) 2) How many mL of Acid (12 M HCl) or base (12 M NaOH) would you need to shift the pH 0.5 increments down?

1. How many grams of NaCl are required to prepare 500. ml of 1.20M NaCl solution?...

1. How many grams of NaCl are required to prepare 500. ml of 1.20M NaCl solution? 2. ) Write the balanced equation for the reaction that occurs when sulfuric acid, H2SO4 is titrated with the base, sodium hydroxide, NaOH. Sodium sulfate, Na2SO4 is the salt produced. 3. SO2 gas is in a 2.0L flask at STP (0 o C, 1 atm). A. Using the Ideal Gas Law, determine the concentration of the gas in moles/L B. Calculate the density of...

Explain how you would make 500 mL of a 20 mM citric acid buffer at a...

Explain how you would make 500 mL of a 20 mM citric acid buffer at a pH of 7.0. You must specify the components that you would use and the masses. Explain what will happen if you add 5 mL of 1.0 M NaOH to this solution.

Show how would you prepare a solution of 500 ml of a 0.15 M acetate buffer....

Show how would you prepare a solution of 500 ml of a 0.15 M acetate buffer. You have available the following: 6M HCl, 4 M acetic, solid sodium acetate, solid sodium hydroxide

How many grams of NaCl are needed to prepare 30 mL of a 0.87 M NaCl...

How many grams of NaCl are needed to prepare 30 mL of a 0.87 M NaCl solution?

Question 6. In titration of 500 ml 0.2 Mn2+ with 0.8 M EDTA (pH = 4),...

Question 6. In titration of 500 ml 0.2 Mn2+ with 0.8 M EDTA (pH = 4), when 200 ml EDTA is added (3 pts) What is the fraction of EDTA in totally unprotonated form? (use the table in lecture notes no calculation is necessary) (3 pts) What is the conditional formation constant? (3 pts) Indicate and calculate the excess and limiting species? (3 pts) What is pMn2+? (3 pts) What are the sources of Mn2+ at beforethe equivalence point, after...