Question

5. You have a dialysis bag containing 15-mL protein solution in 0.20 M NaCl. a. Would it be more efficient to dialyse this solution against i)one 2 L volume of buffer until equilibrium is reached, or to ii)dialyze the solution against one L buffer until equilibrium is reached, the remove the bag and dialyze against another one L of buffer til equilibrium is reached. Assume there is no volume change in the dialysis bag with dialysis. (1 pt) {type answer here } b. What is the concentration of NaCl left in the dialysis bag after condition i? What is the concentration of NaCl left in the dialysis bag after condition ii? (2 pts)

Answer 1

Q) A

Ans. It be more efficient to dialyse this solution against ii) dialyze the solution against one L buffer until equilibrium is reached, the remove the bag and dialyze against another one L of buffer til equilibrium is reached.

Q) B

Ans: concentration of NaCl left in the dialysis 1.5 mM bag after condition i (dilysisng with 2li buffer).

concentration of NaCl left in the dialysis bag after condition ii (serial dilution with 1-1 li buffer)

dialysis with first one lit. concentration 3 mM NaCl.

dialysis with first one lit. concentration 45 Micromolar.

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