Question

immunolog lab questions : please give brief answers. thanks

1. What is one important clinical application of analyzing serum proteins by agarose gel electrophoresis?

2. When you analyzed the sample from your affinity chromography using SDS-PAGE gel, you loaded serum as a control in one well. You then loaded the Flow through in the following well. If the affinity chromatography matrix worked well,

a) which protein band do you expect to be less prominent in the flow through compared to the serum control?

b)why is this protein less prominent?

3. When you analyzed your proteins by SDS-PAGE gel electrophoresis after purification by affinity chromatography, you denatured your proteins by adding b-mercaptoethanol. Based on your SDS gel results; A) did it work? B) why or why not ( how can you tell whether it work or not)?

4. In your analysis of serum proteins using the agarose gel electrophoresis, which proteins migrated slowest in the gel?

a) Albumin

b) Fibrinogen

c) Transferrin

d) IgG

Answer 1

Answer 1) Agarose gel electrophoresis is used for separation of various proteins and DNA molecules on the basis of their size.
Serum protein electrophoresis is a technique of electrophoresis in which proteins present in the serum are separated on the basis of molecular size and charge
One of the important clinical application of analyzing serum proteins by agarose gel electrophoresis is in the detection of various diseases and disorders which occurs due to changes in gamma globulin proteins. And the detection is easy because gamma protein consist of serum immunoglobulin. Which are IgG, IgA, IgM, IgD, and IgE.