Question 2: Describe processes used for the production of viral vectors in mammalian cells. Give a...

Question 2: Describe processes used for the production of viral vectors in mammalian cells. Give a given example of the application of viral vector for treating human disease (analyze a related paper).

Expert Solution

The viral vectors has been modified for the purpose of introducing a material into the cell.

To form viral vectors

Remove the genes in virus that cause disease
Replace it by desired gene

Adeno associated virus AVV

Genome -ds DNA

Capsid-isohedral

Coat - naked

Virion polymerase -negative

Virion diameter -70-90 nm

Genome size -39-38 Kb

The process is divided into three parts

Transfection of packaging cell lines
Virus collection
Transaction into target cell

Transfection

Replication of defective gene is transcfected into plasmids
Nucleic acid sequence packaging
Self activating
Desired targeted genes

Virus collection

Packaging cell produce desired gene with 48-72 hrs
Remove the supernantant and take purified virus gene at -800 C

Transduction

Intergeration of GI into desired gene
This protein of interest is produced

AAV process is by suspension +HSV in manamilian cells

gladiator answered 2 years ago

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