BRIEFLY describe the rationale for doing transfection experiments in mammalian cells.

BRIEFLY describe the rationale for doing transfection experiments in mammalian cells.

Solutions

Expert Solution

Answer-

According to the given question-

Transfection- It is a tool used in genetic engineering to study the gene function and their products by introducing molecules such as Deoxyribonucleic acid (DNA), Ribonucleic acid (RNA) and proteins into other cell where the inserted molecule attached into chromosome of the other cell and leads to change in the cell property. Transfection are of two types depending upon the nature of the genetic materials

Stable transfection- in this type the inserted genetic materials are integrated into the host DNA and and get replicated

Transient transfection- are not integrated into the DNA of host cell but capable of expressing the expression of inserted gene.

Class	Methods	Example
Physical methods	Direct injection Laser-irradiation Electroporation Biolistic particle delivery Magnetic nanoparticle Sonoporation	Gene Gun, phototransfection, Micro-needle, and Magnetofection
Biological	Virus-mediated	Adeno virus, Vaccinia virus, , Herpes simplex virus, Adeno-associated virus.
Chemical	Cationic lipid Cationic polymer Calcium phosphate	polyethyleneimine, calcium phosphate, ,DEAE-dextran, lipofectin, and lipofectamine,

The main rationale behind the transfection experiments in mammalian cells is to study the gene expression, the gene function, the product of genes produced and also for the production of recombinant proteins in mammalian cells. mammalian expression system was used for the production of first products are tissue plasminogen activator now form the different mammalian cells, bacterial cells such as E. coli, yeast cells and some insects are used for the production of different biological products.

Monoclonal antibody production- from the Chinese hamster ovary (CHO) cells.
Urokinase production - Urokinase is a anti-thromboembolic drug which activates plasminogen into plasmin, responsible for degrading fibrin clots.
Follicle stimulating hormone production - produced from the CHO cells.
for production of reporter genes used for the detection of environmental chemicals
Recombinant human Factor IX production for hemophilia B

gladiator answered 1 month ago

Next > < Previous

Related Solutions

Describe the process of rhodopsin signaling in rod cells of the mammalian retina.

Question 2: Describe processes used for the production of viral vectors in mammalian cells. Give a...

Question 2: Describe processes used for the production of viral vectors in mammalian cells. Give a given example of the application of viral vector for treating human disease (analyze a related paper).

Primordial germ cells form in the early mammalian embryo. For either males or females, describe the...

Primordial germ cells form in the early mammalian embryo. For either males or females, describe the events between the formation of the primordial germ cells and their subsequent release as mature gametes. What molecular factors are important during the early embryonic stages, and how are hormones involved post-puberty?(500words)

Briefly describe the advantages of the presence of EACH mammalian organ system: The digestive system, circulatory,...

Briefly describe the advantages of the presence of EACH mammalian organ system: The digestive system, circulatory, respiratory, immune/lymphatic, excretory, endocrine, reproductive, nervous, integumentary, skeletal, and muscular

The cell membranes of mammalian red blood cells are permeable to urea. If red blood cells...

The cell membranes of mammalian red blood cells are permeable to urea. If red blood cells are dropped into a solution of urea that is identical in osmotic pressure (isosmotic) to the cytoplasm of the cells, although the cells do not swell and burst as quickly as when they are dropped simply into pure water, they eventually swell and burst. Explain. Also discuss how you would design a solution into which red cells could be placed without ever swelling. (Hint:...

Identify one specific example each of mass and momentum transport in a mammalian system. Briefly describe...

Identify one specific example each of mass and momentum transport in a mammalian system. Briefly describe each example and identify the driving force and the resistance. In each example, identify a parameter that can change in value, how that parameter is likely to change, and the effects of that change on mass or momentum transfer in your example.

SARS-CoV2 is a coronavirus that infects the cells of the mammalian respiratory epithelium. To replicate it...

SARS-CoV2 is a coronavirus that infects the cells of the mammalian respiratory epithelium. To replicate it needs to take over the host cells secretory pathway to generate essential viral proteins. You are working to try and find a treatment for this virus. Answer all the following questions related to the virus and the secretory system: a) You discover that the virus needs to insert a protein called ‘Spike’ into the plasma membrane for newly formed viral particles to be infectious....

LOUIS PASTEUR and ROBERT KOCH Briefly describe the experiments that each person performed to demonstrate the...

LOUIS PASTEUR and ROBERT KOCH Briefly describe the experiments that each person performed to demonstrate the relationship between the arthropod and the disease agent.

Briefly describe how a Design of Experiments methodology can be used when testing a new pump...

Briefly describe how a Design of Experiments methodology can be used when testing a new pump design that has three variables (number of impeller blades, channel depth, outlet diameter). Why would a factorial design of experiments be better than optimizing each variable independently?

San Fransico Hep B Free Program, briefly describe the program and provide the rationale why you...

San Fransico Hep B Free Program, briefly describe the program and provide the rationale why you believe it will (or will not) be a sustainable health care improvement program.

Subjects