Question

In: Chemistry

1. Besides silica and alumina, can any other stationary phases be utilized for the separation of...

1. Besides silica and alumina, can any other stationary phases be utilized for the separation of chlorophylls?

2. Predict the order of compounds on a TLC for the b-carotene, chlorophyll a and b, and cryptoxanthin pigments found in spinach. Also, predict the order of elution from the column. Explain/justify your answer with polarity arguments.

Solutions

Expert Solution

1. depending upon the compound you want to seperate different stationry phases on TLC are used . commercially available TLC stationary phases are  silica gel, alumina, cellulose, bonded phases, polyamide,etc.., .since silica gel is highly polar in nature most of the compounds can be sepated using it.so it is widely used.

2. order of compounds on TLC from top to bottom is

b-carotene, chlorophyll-a, chlorophyll-b , xanthophyll

elution strength :perfluoroalkane (weakest), hexane, pentane, carbon tetrachloride, benzene/toluene, dichloromethane, diethyl ether, ethyl acetate, acetonitrile, acetone, 2-propanol/n-butanol, water, methanol, triethylamine, acetic acid, formic acid (strongest).


Related Solutions

Please answer this all question : 1. Is there any other factor besides the nephron which...
Please answer this all question : 1. Is there any other factor besides the nephron which control the reabsorption of water and certain molecules for osmoregulation? Substantiate your answer with reasons? . 2. Analyze the specific role of liver in each of the following: 1. Carbohydrate, lipid and protein metabolism, 2. Processing of drugs and hormones, 3. Excretion of bilirubin, 4. Synthesis of bile salts and 5.Activation of vitamin D? .
Is there any other aspect that gets important when dissolving a solute in a liquid besides...
Is there any other aspect that gets important when dissolving a solute in a liquid besides polarity? Answer in greater than 100 words please.
1. Describe stationary phases and strong versus weak mobile phases for Reverse-phase chromatography, Hypophilic Imteraction chromatography,...
1. Describe stationary phases and strong versus weak mobile phases for Reverse-phase chromatography, Hypophilic Imteraction chromatography, and Ion Exchange chromatography. 2. Describe commonly used ion exchange materials and elution modes. 3. Describe “ion-exchange,” “ion-pair,” and “ion” chromatography. Please try to answer ALL questions, thank you!!!!
Besides taking inventory counts, what other steps or controls can be put into place to protect...
Besides taking inventory counts, what other steps or controls can be put into place to protect the physical and financial value of inventories? Note at least 3 steps or controls for this question. You can discuss any physical controls (i.e. non-computer based) as well as automated controls (i.e. computer based). What is the goal for you each control you noted?
1. Besides medication, discuss other factors that are important for an asthmatic to control in order...
1. Besides medication, discuss other factors that are important for an asthmatic to control in order to prevent flare-ups?   2. What are the expected pharmacological actions of the beta2 adrenergic agonists such as albuterol? 3. When educating the patient, the nurse knows that the patient needs to wait _______ minute(s) between inhalations of the same inhaled medication and ________ minutes between inhalations of different inhaled medications. 4. What is the therapeutic range of theophylline? 5. When using a bronchodilator, such...
Discuss the five fundamental phases of projects. Can you think of other areas that should be...
Discuss the five fundamental phases of projects. Can you think of other areas that should be included for projects? In your discussion, use examples from a project you know of or were involved with. Discuss the planning process and steps used to create the project plan. Did those plans align with the general areas/phases of projects? What challenges did you experience? What would you do differently? What did you learn from the experience?
1. Can you think of any other threats to internal validity that are unique in the...
1. Can you think of any other threats to internal validity that are unique in the world? If so, give an example. 2. Give an example in which regression toward the mean might occur? Thank you for your help.
Besides evolutionary processes, is there any other known reason why a virus must target human cells to grow and multiply?
Besides evolutionary processes, is there any other known reason why a virus must target human cells to grow and multiply?
Besides feedback during an evaluation, what other measures can you employ to facilitate employee improvement when...
Besides feedback during an evaluation, what other measures can you employ to facilitate employee improvement when there are noted deficiencies? Offer three options, and explain which you feel would work best, and explain why.
R5-1. An antibody specific for a protein of interest can be utilized in which of the...
R5-1. An antibody specific for a protein of interest can be utilized in which of the following protein separation techniques. a.     Ion-exchange chromatography b.    affinity chromatography c. western blot d.    Edman degradation e.     A and B f.      B and C
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT