Why do we include the DNase step in in vitro transcription?

Why do we include the DNase step in in vitro transcription?

Expert Solution

•In vitro transcription is an artificial production of RNA [may be m-RNA or r-RNA] molecule for various purposes like used in analytical techniques (e.g. hybridization analysis), structural studies (for NMR and X-ray crystallography), in biochemical and genetic studies (e.g. as antisense reagents), and as functional molecules (ribozymes and aptamers).

•For above uses , the transcript required in a pure form . So a step RNA purification is needed. In in-vitro condition the DNA template and newly synthesized RNA is present in same medium [tube], but our interest is only in RNA so we need to cut/ destroy DNA template molecule and purify RNA for this purpose enzyme DNase is added to digest the DNA template .

•The problem is not in in-vivo condition because of nuclear membrane.

gladiator answered 2 years ago

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