Question

how do we do the test for factor 5 or V assay for liver disease ?

Answer 1

Answer :- Factor V ( Cofactor of Thromobokinase) is an anticoagulant produced in liver and converts Prothrombin (II - Inactive) to Thrombin (IIa - Active). Its molecular weight is 330 kilodaltons and plasma half life of 12 - 36 hours. Deficiency of factor V (Labile factor or Proaccelerin) can lead slowing of blood clotting process. The acquired deficiences of facor V are common than its congenital deficiences in newborn. The concentration of factor V in blood can be calculated by Factor V Assay, which is summarised as follows:-

1. Whole blood is collected in light blue top tube buffered with anticoagulant sodium citrate.

2. Mix blood with anticoagulant by gentle inversion.

3. Centrifugation of blood for 5 minutes in stat spin centrifuge.

4. Collect plasma from centrifuge tube and pour it into 4 ml BCS sample cup

5. Spin plasma for 5 minutes in same centrifuge.

6. Collect plasma after centrfuge and tranfer it into newsample cup for analysis.

REFERENCE RANGE - 54 - 90% ( 0 - 1 DAY AGE), 70 - 120% ( 2- 5 DAYS AGE), 80 - 116% ( 6-30 DAYS), 69-111% (31-90 DAYS AGE), 73-109%(91-180 DAYS)