what is the most efficient way to make up a tris buffer at pH 8.5? what...

what is the most efficient way to make up a tris buffer at pH 8.5? what starting compounds and reagents will you use?

Expert Solution

PREPARATION OF TRIS BUFFER IN pH 8.5 :

We need to determine what concentration (molarity) and volume of Tris buffer we want to make. Once we have decided what we are making, calculated the number of moles of Tris that are required by multiplying the molar concentration of buffer by the volume of buffer being made.
moles of Tris = mol/L x L
Next, determined how many grams of Tris this is by multiplying the number of moles by the molecular weight of Tris (121.14 g/mol).
grams of Tris = (moles) x (121.14 g/mol)
Dissolved the Tris into distilled deionized water, 1/3 to 1/2 of our desired final volume.
Mixed in HCl (e.g., 1M HCl) until the pH meter gives us the desired pH for Tris buffer solution.
Diluted the buffer with water to reach the desired final volume of solution.

MATERIALS REQUIRED TO PREPARE TRIS BUFFER :

TRIS powder
Hydrochloric acid
Distilled water
pH meter
Glassware

THE STRUCTURE OF TRIS BUFFER IS :

queen_honey_blossom answered 2 years ago

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