Question

Explain how the solid phase extraction catridges separates the colorant (a potential interference) from the analytes.

Answer 1

A typical cartridge SPE manifold can have up to 24 cartridges. SPE method uses a packed sorbent material in a liquid handling syringe. They are mounted on its extraction manifold which allows many samples to be processed and allowed for an equal number of samples to pass through them simultaneously. SPE cartridges are available with a variety of stationary phases. Each one can separate analytes according to different chemical properties.

From the large volumes of sludge wastes it can seperate the colorant or co-extracted substances that could potentially interfere with the subsequent analysis. It has a prefilter in Cartridges which prevents particulates and micromolecules from reaching the underlying membrane. Filter has four randomly oriented polypropylene fibers that create a really tough path for the liquid to flow.The pore size of the filter decreases with increasing depth, so that is the reason why colorant particles are trapped on the surface and the analyte just flows.

The analytes are usually collected in sample tubes which are inside or below the manifold after they pass through the stationary phase.