Question

1.      Describe an ELISA procedure that you would use to determine if a person was infected with the human immunodeficiency virus, the causative factor of AIDS.

Answer 1

ELISA Procedure is the procedure that determines antibodies in human bodies.It also determines whether these antibodies may causes infectious diseases.Antibodies are proteins that your body produces in response to harmful substances called antigens.ELISA procesure is as follows

1)First a consent sign is necessary

2) Antiseptic, then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood is done by healthcare provider

3)Needle will be placed in one of your veins to draw a sample of blood.the needle will be removed and a small bandage will be placed on your arm When enough blood has been collected

4)The blood sample will be sent to a laboratory for analysis. In the lab, a technician adds the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together.

5)The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react. You may have the condition if the contents of the dish change color.

6)How much change the enzyme causes allows the technician to determine the presence and amount of antibody.

Thus by knowing the amount of antibodies in the human body a decision is made whether a person is infected with HIV virus