Suppose you make TSA plates and inoculate them with Escherichia coli. After incubating the microbe for...

Suppose you make TSA plates and inoculate them with Escherichia coli. After incubating the microbe for 24 hours, you notice nothing grew. What could explain this

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Expert Solution

The TSA medium includes the tryptic soy agar plate with salt sodium chloride and peptones used for the growth of the microrganisms like Ecoli in the culture plates in the laboratory at various temperature ranges of 25⁰C, 40^oC and 45^oC. The growth of the Ecoli bacteria was inhibited at high temperatures. This effect was studied in two phases namely the stationary phase and the log phase. The pressure of the growth of the Ecoli bacteria due to the temperature and the salt stress inhibited the growth of the microorganisms. This is evident in the studies where the bacteria growth was active during the stationary phase at 45^oC as compared to the growth at the same temperature in the log phase where the bacterial growth was declined. The salt concentration in the agar media was kept at 4% and the growth of the bacteria was checked by adding the salt and removing the salt in the medium.

Reference--

Effects of Sublethal Temperature Stresses on the Culturability and Percentage Injury of Escherichia coli grown in the Laboratory Medium Mohd Nizam Lani1 , Jasmin Mohamad1 and Zaiton Hassan2 ---International Journal of Scientific and Research Publications, Volume 4, Issue 11, November 2014 1 ISSN 2250-3153 .

gladiator answered 1 year ago

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