Which of the following enzymes could be used to excise most of the gene for subunit...

Which of the following enzymes could be used to excise most of the gene for subunit 1 of cytochrome c oxidase without fragmenting any of the other genes? [Hint: use the 2 cutters display option.]

BstEII

SgrAI

BglI

AclI

Solutions

Expert Solution

P.denitrificans cyc A and cta DII genes for cytochrome c 550 and cytochrome c oxidase beta subunit I sequence was take from NCBI.

GTCGACATGGGCCTGCCGGTCGGCATGGATGACCTGGACCTGGCCCTGCGCCGCAGCTTCGCACGGAACT
TTCCGCCGCTCGCCGGCTGATTTTCGCCCTGCGACATATTCTCCCTCAGCCGTTCTACGACGTTTTTCCT
GTATTCTGCCCGCTTGGCACATGATAGCCCTGTCAATCGGGAAGCGGAGAGGTCCGTTGCCCGGACATGA
GCGATACCCAAGAGGAAACGCGATGAAGATCAGCATCTATGCCACTCTCGCCGCCATCACCCTCGCCCTG
CCCGCTGCGGCCCAGGATGGCGACGCCGCCAAAGGCGAGAAAGAATTCAACAAGTGCAAGGCTTGCCACA
TGATCCAGGCGCCGGACGGCACCGACATCATCAAGGGCGGCAAGACCGGGCCCAACCTTTACGGCGTCGT
CGGCCGCAAGATCGCCTCGGAGGAGGGCTTCAAATACGGCGAAGGCATCCTCGAGGTCGCCGAAAAGAAC
CCCGACCTGACCTGGACCGAGGCCGACCTGATCGAATACGTCACCGACCCCAAGCCCTGGCTGGTCAAGA
TGACCGACGACAAGGGCGCCAAGACCAAGATGACCTTCAAGATGGGCAAGAACCAGGCCGACGTGGTGGC
CTTCCTGGCCCAGAACTCGCCCGATGCGGGCGGCGACGGCGAGGCTGCGGCCGAGGGCGAATCGAACTGA
TCCCGACGCCTCCGAGATGTCTCGACGGCCCCGCCCCTGCGTGCGACAAAAGCGCGCAGGGGCGGACTTT
TAGTCCTTTCTTCGCATTGCGAGGGGTAGGATTGTGGTCTAGAAACAGGCGAGTCCGTCGGCCTTTGCGC
CGGCCAGCCGCATGTCTAGGGAGTCCACGCATGGCAGACGCAGCCGTTCACGGCCACGGTGACCATCATG
ACACCCGCGGGTTCTTCACCCGCTGGTTCATGTCAACAAACCACAAGGATATCGGTATCCTTTACCTGTT
CACGGCCGGCATCGTCGGCCTGATCTCGGTATGCTTCACCGTCTATATGCGGATGGAACTGCAGCATCCG
GGCGTGCAATACATGTGCCTGGAAGGCGCGCGTCTCATCGCCGACGCCTCGGCGGAATGCACCCCGAACG
GACACCTGTGGAACGTCATGATCACCTACCACGGCGTGCTCATGATGTTCTTCGTCGTGATCCCGGCACT
GTTCGGCGGTTTCGGCAACTATTTCATGCCGCTGCATATCGGCGCCCCGGACATGGCCTTCCCGCGGCTG
AACAACCTCTCCTACTGGATGTATGTCTGCGGCGTGGCCCTGGGCGTCGCCTCGCTGCTGGCGCCGGGCG
GCAACGACCAGATGGGTTCGGGCGTCGGCTGGGTGCTCTACCCGCCGCTCTCGACCACCGAGGCGGGCTA
TTCCATGGACCTGGCGATCTTTGCCGTCCACGTCTCGGGTGCCTCGTCGATCCTGGGCGCGATCAACATC
ATCACCACCTTCCTCAACATGCGCGCACCGGGCATGACGCTGTTCAAGGTGCCGCTGTTTGCCTGGTCGG
TCTTCATCACCGCCTGGCTGATCCTGCTGTCGCTGCCGGTTCTGGCGGGCGCGATCACCATGCTGCTGAT
GGACCGCAACTTCGGCACGCAGTTCTTCGATCCGGCCGGCGGCGGCGACCCGGTGCTTTACCAGCACATC
CTGTGGTTCTTCGGCCATCCCGAGGTCTATATCATCATCCTGCCGGGCTTCGGCATCATCAGCCACGTCA
TCTCGACCTTCGCCAAGAAGCCGATCTTCGGCTACCTGCCGATGGTGCTGGCCATGGCGGCGATCGGCAT
CCTGGGCTTCGTCGTCTGGGCGCACCACATGTACACGGCCGGCATGTCGCTGACCCAGCAGGCCTATTTC
ATGCTGGCGACCATGACCATCGCGGTGCCCACCGGCATCAAGGTCTTCTCGTGGATCGCGACCATGTGGG
GCGGCAGCATCGAGTTCAAGACGCCGATGCTCTGGGCCTTCGGCTTCCTGTTCCTGTTCACCGTCGGCGG
CGTGACCGGCGTGGTGCTGAGCCAGGCGCCGCTGGACCGGGTCTATCACGACACCTATTACGTCGTGGCC
CACTTCCACTACGTGATGTCGCTGGGCGCGGTGTTCGGCATCTTCGCCGGGGTCTATTACTGGATCGGCA
AGATGTCGGGCCGGCAATACCCGGAATGGGCGGGCCAGCTGCATTTCTGGATGATGTTCATCGGCTCGAA
CCTGATCTTCTTCCCGCAGCACTTCCTGGGCCGCCAGGGCATGCCGCGGCGCTATATCGACTATCCGGTC
GAGTTCGCCTATTGGAACAACATCTCGTCGATCGGCGCCTATATCTCCTTCGCGTCCTTCCTGTTCTTCA
TCGGCATCGTGTTCTACACGCTCTTCGCCGGCAAGCGCGTGAACGTGCCGAACTACTGGAACGAGCATGC
CGACACGCTGGAATGGACCCTGCCCTCGCCGCCGCCCGAGCATACCTTCGAGACCCTGCCCAAGCGCGAG
GACTGGGATCGCGCACACGCGCATTGATCCCCGCATTGATCCAACGATGCCCTATGCATGGCATGATGCG
GCCCCGGAGAATTCCGGGGCCGCATGCTTTCGGCTCGAG

by using http://www.restrictionmapper.org/ analysis were done:-

BglI - Positions are 154, 635, 1073, 1807, 2276

BstEII - Position is 898

SgrAI, AclI are non cutter.

SO, Answers are BstEII, BglI.

gladiator answered 1 year ago

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