Question

gene editing

a. How does the enzyme used in CRISPR-Cas9 differ from enzymes used in recombinant DNA technology?

b.Can you predict a potential risk of somatic cell gene editing?

Answer 1

Answer a

1. The cas9 enzyme used in the CRISPR gene editing cleaves the sequence which is complementary to the guide rna sequence whereas in recombinant DNA technology the restriction enzymes cleaves at the pallindromic sequrnce called restriction sites.

2. The cutting of the CRISPR system enzyme is highly specific in comparison to the recombinant DNA technology.

3. The restriction enzymes cannot cleave ss rna whereas crispr enzymes can cleave it

4. Using Crispr we can target many sites on the DNA and it to design an enzyme for that it takes less time and money, whereas in recombinant dna technology, to cut a site different from the restriction sites you need to design the whole new enzyme which require lots of time and money investment.

Answer b

Potential risks of somatic cell gene editing

1. Risk of insertion of the gene at the wrong location

2. Risk of change in the codon sequences which can further affect the expression of the other protein

.3.While using virus for targetting the gene, that would lead to the immune response aginst the vector and can lead to inflammation and organ damage

4.If virus growth becomes uncontrolled then it can affect healthy cells also and can lead to infection

5. It could also affect the reproductive cells which can further affect the next generation